Microchip-based Plasma Separation from Whole Blood via Axial Migration of Blood Cells

• AOTA Arata

  Institute of Microchemical Technology

• TAKAHASHI Susumu

  Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo

• MAWATARI Kazuma

  Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo

• TANAKA Yo

  Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo

• SUGII Yasuhiko

  Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo

• KITAMORI Takehiko

  Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo

抄録

Highly efficient cell-free plasma separation from 200 μL of human whole blood was realized via axial migration of blood cells and cross-flow filtration in a microchip. Although various analyses of small volumes of blood have been reported, a large volume of blood is necessary for obtaining blood cells and plasma for the conventional plasma separation technique of centrifugation. A highly efficient plasma separation method using small volumes of blood without hemolysis is an important issue. We developed a plasma separation method based on a microchip with a filter, which utilizes the axial migration of blood cells observed in blood vessels. Clogging and hemolysis on the filter can be prevented by the axial migration of the blood cells. Using this method, 65% of the plasma from 200 μL of whole blood was successfully separated without hemolysis. When the plasma separation microchip interfaced with a micro-ELISA system was applied to C-reactive protein (CRP) analysis, the CRP concentration obtained by the microchip showed good correlation with that obtained by conventional centrifugation. Total analysis time, including plasma separation, was achieved in only 25 min.

収録刊行物

• Analytical Sciences

  Analytical Sciences 27 (12), 1173-, 2011

  社団法人 日本分析化学会

キーワード

• Analytical Chemistry

詳細情報

• CRID: 1390282679236125440
• NII論文ID: 130004827068
• NII論文ID: 10030445482
• NII書誌ID: AA10500785
• DOI: 10.2116/analsci.27.1173
• ISSN: 13482246
• ISSN: 09106340
• NDL書誌ID: 023335060
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023335060
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.27.1173.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.27.1173/fulltext.html
• Web Site: http://www.jstage.jst.go.jp/article/analsci/27/12/27_12_1173/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012230688
• 本文言語コード: en
• データソース種別:
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