Development of a N-Acetyl-β-D-glucosaminidase (NAG) Assay on a Centrifugal Lab-on-a-compact-disc (Lab-CD) Platform
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- TANAKA Yoshihide
- Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)
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- OKUDA Seira
- Faculty of Pharmaceutical Sciences, Kinki University
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- SAWAI Ayumi
- Faculty of Pharmaceutical Sciences, Kinki University
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- SUZUKI Shigeo
- Faculty of Pharmaceutical Sciences, Kinki University
書誌事項
- タイトル別名
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- Development of a N-Acetyl-.BETA.-D-glucosaminidase (NAG) Assay on a Centrifugal Lab-on-a-compact-disc (Lab-CD) Platform
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抄録
A centrifugal microfluidic platform, which is also known as lab-on-a-compact-disc (Lab-CD), was developed for use as a urinary N-acetyl-β-D-glucosaminidase (NAG) activity assay. In this work, Lab-CD design, centrifugal operations and analytical procedures were established. Automated liquid handling on Lab-CD processes, such as fluid transport, sample metering, mixing, and fluorescence detection are accomplished using a portable Lab-CD system. The linearity of the NAG assay using 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-GlcNAc) was found to be acceptable in the range of 2.5 to 20 U L−1; the relative standard deviations for the fluorescence intensity of eight samples (7.5 U L−1) was 6.4%. Clinical diagnostics is one of the most promising applications for Lab-CD technologies. All the benefits of miniaturization, such as reduced sample requirement, reduced reagent consumption and automation, are realized in this investigation.
収録刊行物
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- Analytical Sciences
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Analytical Sciences 28 (1), 33-, 2012
社団法人 日本分析化学会
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詳細情報 詳細情報について
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- CRID
- 1390001204260961024
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- NII論文ID
- 130004827174
- 10030446146
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- NII書誌ID
- AA10500785
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- COI
- 1:STN:280:DC%2BC387jt1Wnug%3D%3D
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- ISSN
- 13482246
- 09106340
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- NDL書誌ID
- 023399773
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- PubMed
- 22232221
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可