Determination of Histamine in Seafood by Hydrophilic Interaction Chromatography/Tandem Mass Spectrometry

  • YOSHIDA Tatsuo
    Kumamoto Prefectural Institute of Public Health and Environmental Science Department of Chemistry, Kumamoto University
  • HAMADA Hirotoshi
    Kumamoto Prefectural Institute of Public Health and Environmental Science
  • MURAKAWA Hiroshi
    Kumamoto Prefectural Institute of Public Health and Environmental Science
  • YOSHIMOTO Hidekazu
    Kumamoto Prefectural Institute of Public Health and Environmental Science
  • TOBINO Toshiaki
    Kumamoto Prefectural Institute of Public Health and Environmental Science
  • TODA Kei
    Department of Chemistry, Kumamoto University

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Abstract

A simple method was developed to determine histamine, an important compound in chemical food poisoning, by extraction followed by hydrophilic interaction chromatography–tandem mass spectrometry using a hydrophilic column with sulfobetaine-type zwitterion groups. The quantitation range in seafood products was from 0.4 to 200 mg kg−1 for 5 g food samples. Quantitative recoveries were obtained with four types of seafood product. These results agreed well with those from the more complex, conventional HPLC method, which requires sample derivatization with dansyl chloride.

Journal

  • Analytical Sciences

    Analytical Sciences 28 (2), 179-, 2012

    The Japan Society for Analytical Chemistry

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