Rapid Detection of Shiga Toxin-Producing Escherichia coli in Ground Beef by an Immunochromatography Kit in Combination with Short-Term Enrichment and Treatment for Shiga Toxin Release
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- ARAKAWA YUMI
- Nippon Veterinary and Life Science University
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- SAWADA TAKUO
- Nippon Veterinary and Life Science University
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- TAKATORI KOSUKE
- Division of Microbiology, National Institute of Health Sciences
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- LEE KEN-ICHI
- Graduate School of Agricultural and Life Sciences, University of Tokyo
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- HARA-KUDO YUKIKO
- Division of Microbiology, National Institute of Health Sciences
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To establish rapid methods to detect Shiga toxin (Stx)-producing Escherichia coli (STEC) in ground beef samples by using an immunochromatography kit, results of 8-h enrichment in various types of broth with shaking were compared. In pure culture, Stx was detected in the culture of trypticase soy broth (TSB) at 42°C and modified EC broth (mEC) at 36°C from all or most serogroups of O26, O111, O128, O157 and OUT. Ground beef samples inoculated with each serogroup were enriched in TSB at 42°C, mEC at 36°C and mEC with novobiocin (NmEC) at 42°C. Although all conditions led to the successful recovery of each serogroup by the plating method, enrichment in NmEC was relatively superior to the other conditions in the detection of Stx by an immunochromatography kit. These results indicated that the growth of STEC and the release of Stx from cells were different in pure cultures and in culture with ground beef. In addition, polymyxin B treatment for 10 min at 37°C and homogenizing with glass beads enhanced the detection of Stx. From the results, it was suggested that an immunochromatography kit in a combination with enrichment in NmEC at 42°C for 8 h, and treatment with polymyxin B or homogenizing would be a rapid method to detect STEC contamination in ground beef.
収録刊行物
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- Biocontrol Science
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Biocontrol Science 16 (4), 159-164, 2011
日本防菌防黴学会
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詳細情報 詳細情報について
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- CRID
- 1390282679440833664
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- NII論文ID
- 10030447691
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- NII書誌ID
- AA11169621
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- ISSN
- 18840205
- 13424815
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可