Platinum Nanoparticles Suppress Osteoclastogenesis Through Scavenging of Reactive Oxygen Species Produced in RAW264.7 Cells

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Author(s)

    • HASEGAWA Tomokazu
    • Department of Pediatric Dentistry, Faculty of Dentistry, Tokushima University
    • TANIGUCHI Yumi
    • Department of Pediatric Dentistry, Hokkaido University Graduate School of Dental Medicine
    • DEYAMA Yoshiaki
    • Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine
    • KOSHIRO Ken-ichi
    • Department of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine
    • SANO Hidehiko
    • Department of Restorative Dentistry, Hokkaido University Graduate School of Dental Medicine
    • SUZUKI Kuniaki
    • Department of Molecular Cell Pharmacology, Hokkaido University Graduate School of Dental Medicine
    • INOUE Nobuo
    • Department of Gerodontology, Hokkaido University Graduate School of Dental Medicine

Abstract

Recent research has shown that platinum nanoparticles (nano-Pt) efficiently quench reactive oxygen species (ROS) as a reducing catalyst. ROS have been suggested to regulate receptor activator of NF-<I>κ</I>B ligand (RANKL)-stimulated osteoclast differentiation. In the present study, we examined the direct effects of platinum nano-Pt on RANKL-induced osteoclast differentiation of murine pre-osteoclastic RAW 264.7 cells. The effect of the nano-Pt on the number of osteoclasts was measured and their effect on the mRNA expression for osteoclast differentiation was assayed using real-time PCR. Nano-Pt appeared to have a ROS-scavenging activity. Nano-Pt decreased the number of osteoclasts (2+ nuclei) and large osteoclasts (8+ nuclei) in a dose-dependent manner without affecting cell viability. In addition, this agent significantly blocked RANKL-induced mRNA expression of osteoclastic differentiation genes such as c-fms, NFATc1, NFATc2, and DC-STAMP as well as that of osteoclast-specific marker genes including MMP-9, Cath-K, CLC7, ATP6i, CTR, and TRAP. Although nano-Pt attenuated expression of the ROS-producing NOX-family oxidases, Nox1 and Nox4, they up-regulated expression of Nox2, the major Nox enzyme in macrophages. These findings suggest that the nano-Pt inhibit RANKL-stimulated osteoclast differentiation via their ROS scavenging property. The use of nano-Pt as scavengers of ROS that is generated by RANKL may be a novel and innovative therapy for bone diseases.

Journal

  • Journal of Pharmacological Sciences

    Journal of Pharmacological Sciences 117(4), 243-252, 2011-12-20

    The Japanese Pharmacological Society

References:  39

Codes

  • NII Article ID (NAID)
    10030453660
  • NII NACSIS-CAT ID (NCID)
    AA11806667
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13478613
  • NDL Article ID
    023386010
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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