Use of Salivary Ammonia Concentration for the Evaluation of Total Oral Bacterial Counts <BR>of the Independent Elderly

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  • 唾液中アンモニアの高齢者における口腔内細菌数評価への応用

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Abstract

The purpose of this study is to propose a convenient method to evaluate total oral bacterial count based on salivary ammonia concentrations by independent elderly.<BR>The Subjects were 146 elderly (mean age 71.7 years) living in Himeji City who had agreed to a study of their oral health behavior, and check ups. After rinsing with 3 ml of distilled water for 10 sec, the mouth rinsed solution (MRS) was then collected as an alternative saliva sample. The total bacterial count and the ammonia concentrations from the MRS were measured as an indicator of their oral cleanliness. The total bacterial count in the MRS was measured by real-time PCR methods and the ammonia concentrations were measured by using ammonia test paper (Ami-check\_hTM).<BR>When it was assessed by logarithmic conversion, the concentrations of ammonia from MRS correlated positively with the total bacterial count (r=0.577) with a statistical significance at p<0.01.<BR>The ammonia production of oral and opportunistic microorganisms was examined with 40 standard microbial strains. In 23 species 31 strains of oral bacteria, 21 species and 26 strains produced ammonia. The most active bacteria was \_iPorphyromonas gingivalis. In addition, 6 species of the 9 strains of opportunistic bacterium, 5 species and 7 strains including \_iPseudomonas aeruginosa, \_iKlebsiella pneumoniae et al. produced ammonia.<BR>From these studies, it is suggested that the concentrations of ammonia from the MRS are useful indicators of oral cleanliness.

Journal

  • Ronen Shika Igaku

    Ronen Shika Igaku 25 (4), 367-374, 2011

    Japanese Society of Gerodontology

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