Structural Basis for mRNA Surveillance by Archaeal Pelota and GTP-bound EF1α Complex
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- KOBAYASHI Kan
- Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo
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- ISHITANI Ryuichiro
- Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo
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- NUREKI Osamu
- Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo
Bibliographic Information
- Other Title
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- 古細菌由来Pelota·EF1α·GTP複合体によるmRNA品質管理機構の構造基盤
- 古細菌由来Pelota・EF1α・GTP複合体によるmRNA品質管理機構の構造基盤
- コ サイキン ユライ Pelota ・ EF1a ・ GTP フクゴウタイ ニ ヨル mRNA ヒンシツ カンリ キコウ ノ コウゾウ キバン
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Abstract
No-go decay (NGD) and Nonstop decay (NSD) are mRNA surveillance pathways that detect the stalled ribosome containing the defective mRNA. In archaea, archaeal Pelota (aPelota) associates with archaeal elongation factor 1α (aEF1α) to act in the mRNA surveillance pathway. Here, we present the complex structure of aPelota and GTP-bound aEF1α determined at 2.3 Å resolution. Notably, the aPelota·aEF1α·GTP complex structurally resembles the tRNA·EF-Tu complex bound to the ribosome. Combined with the functional analysis in yeast, our findings provide structural insights into how aPelota·aEF1α·GTP complex detects the stalled ribosome and triggers NGD and NSD.<br>
Journal
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- Seibutsu Butsuri
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Seibutsu Butsuri 52 (4), 182-185, 2012
The Biophysical Society of Japan General Incorporated Association
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Details 詳細情報について
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- CRID
- 1390282681510924288
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- NII Article ID
- 10030494636
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- NII Book ID
- AN00129693
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- COI
- 1:CAS:528:DC%2BC38XhtFOrt7jE
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- ISSN
- 13474219
- 05824052
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- NDL BIB ID
- 023910251
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed