古細菌由来 Pelota・EF1α・GTP 複合体によるmRNA品質管理機構の構造基盤  [in Japanese] Structural Basis for mRNA Surveillance by Archaeal Pelota and GTP-bound EF1α Complex  [in Japanese]

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Author(s)

    • 小林 幹 KOBAYASHI Kan
    • 東京大学大学院理学系研究科生物化学専攻 Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo
    • 石谷 隆一郎 ISHITANI Ryuichiro
    • 東京大学大学院理学系研究科生物化学専攻 Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo
    • 濡木 理 NUREKI Osamu
    • 東京大学大学院理学系研究科生物化学専攻 Department of Biophysics and Biochemistry, Graduate School of Science, the University of Tokyo

Abstract

No-go decay (NGD) and Nonstop decay (NSD) are mRNA surveillance pathways that detect the stalled ribosome containing the defective mRNA. In archaea, archaeal Pelota (aPelota) associates with archaeal elongation factor 1α (aEF1α) to act in the mRNA surveillance pathway. Here, we present the complex structure of aPelota and GTP-bound aEF1α determined at 2.3 Å resolution. Notably, the aPelota·aEF1α·GTP complex structurally resembles the tRNA·EF-Tu complex bound to the ribosome. Combined with the functional analysis in yeast, our findings provide structural insights into how aPelota·aEF1α·GTP complex detects the stalled ribosome and triggers NGD and NSD.<br>

Journal

  • Seibutsu Butsuri

    Seibutsu Butsuri 52(4), 182-185, 2012-07-25

    The Biophysical Society of Japan General Incorporated Association

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Codes

  • NII Article ID (NAID)
    10030494636
  • NII NACSIS-CAT ID (NCID)
    AN00129693
  • Text Lang
    JPN
  • Article Type
    REV
  • ISSN
    05824052
  • NDL Article ID
    023910251
  • NDL Call No.
    Z18-49
  • Data Source
    CJP  NDL  J-STAGE 
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