蛍光・化学発光反応に基づく高選択的検出法の開発と生体分析への応用  [in Japanese] Development of Selective Detection Methods for Pharmaceutical and Biological Compounds Based on Fluorescence and Chemiluminescence Techniques and Its Application for Biomedical Analyses  [in Japanese]

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Author(s)

    • 岸川 直哉 KISHIKAWA Naoya
    • 長崎大学大学院医歯薬学総合研究科生命薬科学専攻 Graduate School of Biomedical Science, Nagasaki University

Abstract

生体内に存在する医薬品やバイオマーカーの濃度を測定することは,治療効果の確認や疾患の診断のために重要である.しかしながら,生体に由来する試料中には<ruby><rb>夾</rb><rp>(</rp><rt>きょう</rt><rp>)</rp></ruby>雑物となる生体成分が数多く存在し,これらが測定対象の検出を妨害するという問題点が存在している.著者らは,複雑な生体成分が共存する生体内から,微量の医薬品やバイオマーカーのみを特異的に検出するために,測定対象化合物に特徴的な構造あるいは性質を利用する高選択的かつ高感度な蛍光・化学発光分析法の開発を行い,生体分析の分野においてその有用性を明らかにしてきた.本論文ではその中でも中心的な二つの手法,(1)パラジウムカップリング反応に基づくアリールハライド及び末端二重結合の選択的蛍光誘導体化法及び(2)キノンの選択的蛍光・化学発光定量法について紹介する.

In order to provide therapeutic advice or diagnosis, it is important to determine the concentration of a pharmaceutical agent or biomarker in biological fluids. However, it is difficult to determine a target analyte selectively due to interferences derived from a large number of co-existing biological components. In order to overcome this problem, we have developed selective detection methods for pharmaceutical agent or biomarker in the presence of biological components using a specific structure or characteristic of the target analyte. Among related studies, this review describes two principle methods : namely (1) selective fluorescence derivatization methods for aryl halide or terminal double bond based on palladium coupling reaction, and (2) selective detection methods for quinone by chemiluminescence and fluorescence reactions of utilizing the specific characteristics of quinone.

Journal

  • BUNSEKI KAGAKU

    BUNSEKI KAGAKU 61(7), 583-590, 2012-07-05

    The Japan Society for Analytical Chemistry

References:  35

  • <no title>

    YAMAGUCHI M.

    Modern Derivatization Methods for Separation Sciences : Reagent for FL Detection 99, 1999

    Cited by (1)

  • <no title>

    UCHIYAMA S.

    Biomed. Chromatogr. 15, 295, 2001

    Cited by (1)

  • <no title>

    GARCIA-CAMPANA A. M.

    Historical evolution of chemiluminescence 2, 2001

    Cited by (1)

  • <no title>

    KURODA N.

    Chemiluminescence detection in liquid chromatography 393, 2001

    Cited by (1)

  • <no title>

    MIYAURA N.

    Chem. Rev. 95, 2457, 1995

    Cited by (1)

  • <no title>

    MIZOROKI T.

    Bull. Chem. Soc. Jpn. 44, 581, 1971

    Cited by (1)

  • <no title>

    HECK R. F.

    J. Org. Chem. 37, 2320, 1972

    Cited by (1)

  • <no title>

    KURODA N.

    Luminescence 14, 361, 1999

    Cited by (1)

  • <no title>

    KURODA N.

    J. Chromatogr. A 1066, 119, 2005

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    Anal. Bioanal. Chem. 386, 719, 2006

    Cited by (1)

  • <no title>

    HAMMAD S. F.

    Biomed. Chromatogr. 21, 1030, 2007

    Cited by (1)

  • <no title>

    ADUTWUM L. A.

    Anal. Bioanal. Chem. 398, 823, 2010

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    J. Chromatogr. A 1216, 6873, 2009

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    J. Chromatogr. A 1218, 3002, 2011

    Cited by (1)

  • <no title>

    SHEARER M. J.

    Lancet 345, 229, 1995

    Cited by (1)

  • <no title>

    MINOTTI G.

    Pharmacol. Rev. 56, 185, 2004

    Cited by (1)

  • <no title>

    ASSIMOPOULOUA A. N.

    Food Chem. 87, 433, 2004

    Cited by (1)

  • <no title>

    MOTOYAMA Y.

    J. Health Sci. 55, 845, 2009

    Cited by (1)

  • <no title>

    TAGUCHI K.

    Free Radic. Biol. Med. 44, 1645, 2008

    Cited by (1)

  • <no title>

    TAGUCHI K.

    J. Health Sci. 47, 571, 2001

    Cited by (1)

  • <no title>

    YAMAGUCHI S.

    Anal. Chim. Acta 665, 74, 2010

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    Anal. Bioanal. Chem. 393, 1337, 2009

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    Anal. Bioanal. Chem. 400, 381, 2011

    Cited by (1)

  • <no title>

    AHMED S.

    J. Chromatogr. A 1133, 76, 2006

    Cited by (1)

  • <no title>

    AHMED S.

    Anal. Chim. Acta 591, 148, 2007

    Cited by (1)

  • <no title>

    AHMED S.

    Talanta 78, 94, 2009

    Cited by (1)

  • <no title>

    AHMED S.

    J. Chromatogr. A 1216, 3977, 2009

    Cited by (1)

  • <no title>

    AHMED S.

    Talanta 85, 230, 2011

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    J. Chromatogr. A 1057, 83, 2004

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    Chemosphere 64, 834, 2006

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    Talanta 85, 809, 2011

    Cited by (1)

  • <no title>

    KISHIKAWA N.

    Talanta 81, 1852, 2010

    Cited by (1)

  • <no title>

    OHKURA Y.

    J. Chromatogr. B 659, 85, 1994

    DOI Cited by (7)

  • <no title>

    SUZUKI A.

    J. Organomet. Chem. 576, 147, 1999

    Cited by (21)

  • <no title>

    OVERVAD K

    Eur. J. Clin. Nutr. 53, 764, 1999

    DOI Cited by (3)

Codes

  • NII Article ID (NAID)
    10030494951
  • NII NACSIS-CAT ID (NCID)
    AN00222633
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    05251931
  • NDL Article ID
    023897728
  • NDL Call No.
    Z17-9
  • Data Source
    CJP  NDL  IR  J-STAGE 
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