Interstitial cells of Cajal in the gastrointestinal musculature of Wjic c-kit mutant mice

• Iino Satoshi

  Department of Morphological and Physiological Sciences, University of Fukui, Japan

• Horiguchi Satomi

  Department of Morphological and Physiological Sciences, University of Fukui, Japan

• Horiguchi Kazuhide

  Department of Morphological and Physiological Sciences, University of Fukui, Japan

抄録

Interstitial cells of Cajal (ICC) generate electrical rhythmicity and transduce neural signals in the gastrointestinal musculature. ICC express the proto-oncogene c-kit, a receptor tyrosine kinase, and are identified morphologically by c-Kit immunoreactivity. The c-kit gene is allelic with the murine white-spotting locus W, and mutations of c-kit are known as W mutations. W mutations affect various developmental aspects of hematopoietic cells, germ cells, melanocytes, mast cells and ICC. We examined W^jic/W^jic mutant mice that have a mutation in the tyrosine kinase domain resulting in severe loss of protein function. W^jic/W^jic homozygotes exhibited white coats and black eyes. The gross morphology of the gastrointestinal tract showed no abnormality in mutant mice other than a forestomach papilloma. In the stomach, intramuscular ICC (ICC-IM) were missing, and myenteric ICC (ICC-MY) were reduced in number. In the small intestine, the number of ICC-MY was severely reduced; however there was a normal distribution of deep muscular plexus ICC (ICC-DMP). In the cecum, the numbers of ICC-IM and ICC-MY were severely depleted. ICC-IM were almost entirely absent in the colon, whereas ICC-MY loss was restricted to the distal colon. Patterns of ICC deficiency were generally similar between W^jic/W^jic mice and W/W^v mutants, which lack a specific type of ICC. The enteric nervous system of the mutant mice appeared normal. From these findings, we conclude that W^jic/W^jic mice represent a distinct, novel genotype resulting in a lack of a specific type of ICC in the gastrointestinal musculature.<br>

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ｓｍｏｏｔｈ　Ｍｕｓｃｌｅ　Ｒｅｓｅａｒｃｈ

  Ｊｏｕｒｎａｌ　ｏｆ　Ｓｍｏｏｔｈ　Ｍｕｓｃｌｅ　Ｒｅｓｅａｒｃｈ 47 (3/4), 111-121, 2011

  日本平滑筋学会

キーワード

• <i>W</i> mutant
• c-Kit
• interstitial cells of Cajal
• gastrointestinal tract

詳細情報

• CRID: 1390001205058092160
• NII論文ID: 10030498082
• NII書誌ID: AN10364204
• DOI: 10.1540/jsmr.47.111
• ISSN: 18848796
• ISSN: 09168737
• Web Site: http://www.jstage.jst.go.jp/article/jsmr/47/3+4/47_3+4_111/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012152569
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
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