Endothelial Gab1 Deletion Accelerates Angiotensin II-Dependent Vascular Inflammation and Atherosclerosis in Apolipoprotein E Knockout Mice

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Author(s)

    • HIGUCHI Kaori
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • NAKAOKA Yoshikazu
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • SHIOYAMA Wataru
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • ARITA Yoh
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • HASHIMOTO Takahiro
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • YASUI Taku
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • IKEOKA Kuniyasu
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
    • KURODA Tadashi
    • Department of Advanced Cardiovascular, Osaka University Graduate School of Medicine
    • MINAMI Takashi
    • Laboratory for Vascular Biology, Research Center for Advanced Science and Technology, University of Tokyo
    • NISHIDA Keigo
    • Laboratory for Cytokine Signaling, RIKEN Research Center for Allergy and Immunology
    • FUJIO Yasushi
    • Department of Clinical Pharmacology and Pharmacogenomics, Osaka University Graduate School of Pharmaceutical Sciences
    • SHIRAI Mikiyasu
    • Department of Cardiac Physiology, National Cerebral and Cardiovascular Center Research Institute
    • MOCHIZUKI Naoki
    • Department of Cell Biology, National Cerebral and Cardiovascular Center Research Institute
    • KOMURO Issei
    • Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine

Abstract

<b><i>Background:</i></b> Docking protein Grb2-associated binder 1 (Gab1) has critical roles in signal transduction of various growth factors, cytokines, and numerous other molecules. Our previous reports show that Gab1 is essential for postnatal angiogenesis through the analysis of endothelium-specific Gab1 knockout (Gab1ECKO) mice. However, the role of Gab1 in atherosclerosis remains unknown. The aim of the present study was to elucidate the role of endothelial Gab1 in vascular inflammation and atherosclerosis. <b><i>Methods and Results:</i></b> We intercrossed Gab1ECKO mice with apolipoprotein E (ApoE) knockout (ApoEKO) mice. Six-month-old male ApoEKO/Gab1ECKO and littermate control (ApoEKO) mice were treated with angiotensin II (AngII) via an osmotic infusion mini-pump. After AngII treatment, ApoEKO/Gab1ECKO mice showed significantly enhanced atherosclerosis and aneurysm formation compared with control mice. The production of proinflammatory cytokines in the aorta was significantly enhanced in ApoEKO/Gab1ECKO mice compared with control mice. Furthermore, the expression levels of Krüppel-like factor (KLF) 2 (KLF2) and KLF4, key transcription factors for endothelial homeostasis, were significantly reduced in the aortic endothelium of ApoEKO/Gab1ECKO mice compared with those of control mice. Consistently, both vascular cell adhesion molecule-1 expression and macrophage infiltration on the aortic walls were enhanced in ApoEKO/Gab1ECKO mice compared with control mice. <b><i>Conclusions:</i></b> Collectively, endothelial Gab1 deletion accelerates AngII-dependent vascular inflammation and atherosclerosis on <i>ApoE</i>-null background presumably in association with downregulation of KLF2 and KLF4.  (<i>Circ J</i> 2012; <b>76:</b> 2031–2040)<br>

Journal

  • Circulation Journal

    Circulation Journal 76(8), 2031-2040, 2012-07-25

    The Japanese Circulation Society

References:  40

Cited by:  1

Codes

  • NII Article ID (NAID)
    10030505176
  • NII NACSIS-CAT ID (NCID)
    AA11591968
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13469843
  • Data Source
    CJP  CJPref  J-STAGE 
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