Co-culture Devices for in vitro Monitoring of Neural Transplantation Processes

• Ito Hiroki

  Graduate School of Frontier Sciences, The University of Tokyo

• Shimba Kenta

  Graduate School of Frontier Sciences, The University of Tokyo

• Takeuchi Akimasa

  Graduate School of Frontier Sciences, The University of Tokyo

• Takayama Yuzo

  Graduate School of Information Science and Technology, The University of Tokyo

• Kotani Kiyoshi

  Graduate School of Frontier Sciences, The University of Tokyo

• Jimbo Yasuhiko

  Graduate School of Frontier Sciences, The University of Tokyo

Bibliographic Information

Other Title

• 神経細胞移植　ｉｎ　ｖｉｔｒｏ　評価のための共培養デバイス試作
• シンケイ サイボウ イショク in vitro ヒョウカ ノ タメ ノ キョウ バイヨウ デバイス シサク

Abstract

Transplantation of stem cell-derived neurons into the damaged neuronal tissue is one of the promising methods for the recovery of neuronal functions. However, little is known about the signal transmission between transplanted stem cell-derived neuronal networks and the host brain tissue during recovery. In this study, we developed microelectrode-array (MEA) substrates with multiple micro-chambers for cell cultures. Each micro-chamber consisted of 4 cell-plating spots with interconnecting conduits fabricated by polydimethylsiloxane (PDMS) lithography. The conduits had the tunneling structures, the height of which was approximately 6 μm, through which only neurites could grow into adjacent cell-plating spots. We cultured mouse cortical neurons in the subset of cell-plating spots, and stem cell-derived neurons were injected into the residual spots 7 days after starting incubation of primary cultures. Intracellular calcium transients as well as electrical activity were detected from both primary cultured-cells. And extracellular electrical activities were recorded from both primary cultured-cells and stem cell-derived neurons in the co-cultures. These results suggested that our MEA-based co-culture system would be a useful tool for in vitro monitoring of transplantation processes of stem cell-derived neurons into host neuronal tissue.

Journal

• IEEJ Transactions on Electronics, Information and Systems

  IEEJ Transactions on Electronics, Information and Systems 131 (11), 1983-1989, 2011

  The Institute of Electrical Engineers of Japan

Keywords

• microelectrode array
• neuronal network
• patterned culture
• microstructure
• tissue engineering

Details

• CRID: 1390282679583626368
• NII Article ID: 10030528241
• NII Book ID: AN10065950
• DOI: 10.1541/ieejeiss.131.1983
• ISSN: 13488155
• ISSN: 03854221
• NDL BIB ID: 11293566
• Web Site: http://id.ndl.go.jp/bib/11293566
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I11293566
• Web Site: http://www.jstage.jst.go.jp/article/ieejeiss/131/11/131_11_1983/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed