Epithelial-mesenchymal interaction reduces inhibitory effects of fluoride on proliferation and enamel matrix expression in dental epithelial cells

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Author(s)

    • YAMADA Aya
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • IWAMOTO Tsutomu
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • FUKUMOTO Emiko
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • ARAKAKI Makiko
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • MIYAMOTO Ryoko
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • SUGAWARA Yu
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • KOMATSU Hideji
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • NAKAMURA Takashi
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry
    • FUKUMOTO Satoshi
    • Division of Pediatric Dentistry, Department of Oral Health and Development Sciences, Tohoku University Graduate School of Dentistry

Abstract

AIM: Fluoride, well known as a specific and effective caries prophylactic agent, also affects the differentiation and function of ameloblasts. High dose sodium fluoride (NaF) induces enamel hypoplasia, also called enamel fluorosis, whereas the size and form of teeth except the enamel are not changed with its treatment. We examined the effects of fluoride on dental epithelium proliferation and differentiation using co-cultures of dental epithelial and mesenchymal cells. METHODS: Cultures of the dental epithelial cell line SF2 and dental mesenchymal cell line mDP were performed, as well as co-cultures. Enamel matrix expression in SF2 cells treated with NaF was analyzed by RT-PCR, while cell proliferation was examined using a trypan blue dye exclusion method and BrdU incorporation findings. The effects of NaF on NT-4-induced ERK1/2 phosphorylation were analyzed by western immunoblotting. RESULTS: Neurotrophic factor NT-4 induced enamel matrix expression, which was inhibited in the presence of NaF. Similar results were observed in regard to SF2 cell proliferation, but not with mDP cells. The levels of proliferation and ameloblastin expression in SF2-GFP cells co-cultured with mDP in the presence of NaF were lower as compared to those in SF2 cells cultured alone. CONCLUSION: Our results indicate that dental epithelial cells co-cultured with dental mesenchymal cells are resistant to the inhibitory effects of NaF on proliferation and ameloblastin expression. They also suggest that the dental fluorosis phenotype may affect enamel, but not tooth size or shape, because of rescue of the inhibitory effects of NaF by culturing with dental mesenchymal cells.

Journal

  • Pediatric Dental Journal

    Pediatric Dental Journal 22(1), 55-63, 2012-03-30

    The Japanese Society of Pediatric Dentistry

References:  36

Codes

  • NII Article ID (NAID)
    10030558189
  • NII NACSIS-CAT ID (NCID)
    AA10809637
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09172394
  • Data Source
    CJP  J-STAGE 
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