Differences in intron-mediated enhancement of gene expression by the first intron of cytosolic superoxide dismutase gene from rice in monocot and dicot plants

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Abstract

Introns have an enhancing effect on gene expression known as intron-mediated enhancement in various organisms, including plants. Although the mechanism of the enhancement is largely unknown, most enhancing introns are first introns. In this study, we examined whether the first intron of rice superoxide dismutase <i>sodCc2</i> gene has an enhancing effect in rice and other plant species. A transient expression assay revealed that the <i>sodCc2</i> intron elevated reporter gene expression in rice, wheat, and maize, but not in <i>Arabidopsis</i> and tobacco, indicating that the <i>sodCc2</i> intron has an enhancing effect in monocot but not in dicot plants. To find the putative signal sequences responsible for the enhancement, we carried out an <i>in silico</i> search and found two motifs conserved among the <i>sodCc2</i> intron and 11 enhancing introns previously known in rice. The motifs contain a consensus sequence, GATCTG, which also exists in the conserved motif found in <i>Arabidopsis</i> enhancing introns. Our results suggest that common motifs are conserved between rice and <i>Arabidopsis</i> enhancing introns.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29(1), 115-119, 2012-03-25

    Japanese Society for Plant Cell and Molecular Biology

References:  21

Codes

  • NII Article ID (NAID)
    10030561667
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    13424580
  • NDL Article ID
    023568264
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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