Biomolecule Analyses in an Open-Tubular Capillary Chromatography Using Ternary Mixed Carrier Solvents with Chemiluminescence Detection

• TAKAHASHI Naoya

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• MASUHARA Yuji

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• JINNO Naoya

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• HASHIMOTO Masahiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• TSUKAGOSHI Kazuhiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

Abstract

We examined the elution behavior of isoluminol isothiocyanate (ILITC)-labeled biomolecules (α-amino acids, peptides, and proteins) in an open-tubular capillary chromatography system using an untreated fused-silica capillary tube and a water–acetonitrile–ethyl acetate mixture carrier solution. Such an open-tubular capillary chromatography is called “tube radial distribution chromatography (TRDC)” for convenience. A mixture of ILITC and ILITC-labeled biomolecules was analyzed using TRDC with chemiluminescence detection that provided simple instrument without a light source and complex optical devises. The ILITC and the labeled twenty α-amino acids were separated, in this order or the reverse order, or not separated with an organic solvent-rich and water-rich carrier solution. Their elution behavior was considered to be of hydrophilic or hydrophobic nature of ILITC and the labeled α-amino acids. The ILITC and the labeled protein, alcohol dehydrogenase and bovine serum albumin, were separated in this order with an organic solvent-rich carrier solution, while they were eluted in the reverse order with a water-rich carrier solution, based on the TRDC separation performance. The TRDC system worked with the untreated open-tubular capillary tube not using any specific capillary tubes, such as coated, packed, or monolithic.

Journal

• Analytical Sciences

  Analytical Sciences 28 (4), 351-351, 2012

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390282679235933696
• NII Article ID: 130004827215
• NII Article ID: 10030570607
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.28.351
• COI: 1:STN:280:DC%2BC38rksFyisw%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 023556274
• PubMed: 22498461
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023556274
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.28.351.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.28.351/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/28/4/28_4_351/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed