トランスポゾンを指標にしたコシヒカリ品種内の遺伝的差異  [in Japanese] Genetic differences within the japonica rice variety, Koshihikari, indicated by transposons  [in Japanese]

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Author(s)

    • 貴島 祐治 KISHIMA Yuji
    • 北海道大学大学院農学研究院・植物育種学研究室 Laboratory of Plant Breeding, Research Faculty of Agriculture, Hokkaido University
    • 堀田 夕夏 HOTTA Yuka
    • 北海道大学大学院農学研究院・植物育種学研究室 Laboratory of Plant Breeding, Research Faculty of Agriculture, Hokkaido University
    • 石黒 聖也 [他] ISHIGURO Seiya
    • 北海道大学大学院農学研究院・植物育種学研究室 Laboratory of Plant Breeding, Research Faculty of Agriculture, Hokkaido University
    • 山村 和照 YAMAMURA Kazuteru
    • 北海道大学大学院農学研究院・植物育種学研究室 Laboratory of Plant Breeding, Research Faculty of Agriculture, Hokkaido University
    • 佐野 芳雄 SANO Yoshio
    • 北海道大学大学院農学研究院・植物育種学研究室 Laboratory of Plant Breeding, Research Faculty of Agriculture, Hokkaido University

Abstract

特定の品種の中の遺伝変異やその発生過程については,殆ど知見がない.本研究では全国15府県から集められたコシヒカリ86集団を材料に,遺伝的な変異についてトランスポゾンの挿入位置を指標に調査した.3種のトランスポゾンの挿入位置を調べると,4集団の例外をのぞいて,コシヒカリ集団間での多型はきわめて少なく,品種の遺伝的同一性が保持されていることが示された.しかし,トランスポゾン <i>mPing</i>を指標にした場合,多くの多型が現れた.多数を占めたのは,コシヒカリの親である農林1号と農林22号にはない固有バンドからなる多型で, <i>mPing</i>がコシヒカリで転移していることを示すものである.その他の多型には,新潟以外の集団にのみ存在し,新潟由来の集団にはない2つのバンドが検出され,コシヒカリを新潟タイプと非新潟タイプ(福井タイプ)で明瞭に分けた.2つの多型バンドは両親に1つずつ存在するので,コシヒカリの育成過程で発生した遺伝的分離に基づくと考えられる.新潟では福井県農業試験場から分譲された育成過程のコシヒカリを独自で選抜したとされ,この期間に新潟タイプと福井タイプのコシヒカリの間に遺伝的な差異が発生したと思われる.<br>

Little is known about genetic variation, which follows the establishment of plant variety, and its accumulation process. Here, we collected samples from a total of 86 populations of a popular japonica rice variety, Koshihikari, from 15 prefectures in Japan and investigated genetic variation among these samples. In plants, insertion sites of transposable elements are of strong indications of genetic polymorphisms among different strains of the same species. Particularly, rice genomes contain at least seven potentially active transposable elements that could give rise to <i>de novo</i> insertions and mutations. In this study, transposon display (TD) experiments were performed with four transposable elements, which showed different banding profiles between the parents of Koshihikari, Norin 1 and Norin 22. In TDs involving three elements, 82 samples from the 86 populations examined showed common patterns, implying that these populations share an identity as Koshihikari. In contrast, TD involving the transposon <i>mPing</i> exhibited a number of polymorphisms among the 82 populations. These polymorphisms include population specific bands, which may result from recent <i>de novo</i> transpositions of <i>mPing</i>, indicating the activity of <i>mPing</i> in Koshihikari. Interestingly, the other polymorphisms due to two bands, A1 and A23, are able to classify Koshihikari populations into two types; the two bands distinguish Niigata or non-Niigata (Fukui) types where the samples originated. The A1 and A23 bands are derived from the Koshihikari parents, Norin 1 or Norin 22, respectively. The results suggest that the Koshihikari variety consists of two major populations (Niigata and Fukui types) with partially different genome structures that might be established during the breeding processes.<br>

Journal

  • Breeding Research

    Breeding Research 12(3), 81-86, 2010-09-01

    Japanese Society of Breeding

References:  21

Codes

  • NII Article ID (NAID)
    10030690483
  • NII NACSIS-CAT ID (NCID)
    AA11317194
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    13447629
  • NDL Article ID
    10834454
  • NDL Source Classification
    ZR7(科学技術--農林水産--農産)
  • NDL Call No.
    Z74-B467
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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