Reactivity of Direct Assays for Low-Density Lipoprotein (LDL) Cholesterol Toward Charge-Modified LDL in Hypercholesterolemia

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Author(s)

    • ZHANG Bo
    • Department of Biochemistry, Fukuoka University School of Medicine
    • KAWACHI Emi
    • Department of Cardiology, Fukuoka University School of Medicine
    • MATSUNAGA Akira
    • Department of Laboratory Medicine, Fukuoka University School of Medicine
    • NODA Keita
    • Department of Cardiology, Fukuoka University School of Medicine
    • YOSHINAGA Kazuhiko
    • Department of Research Laboratory for Social Medicine, Fukuoka University School of Medicine
    • SAKU Keijiro
    • Department of Cardiology, Fukuoka University School of Medicine

Abstract

<b><i>Background:</i></b> The aim of the present study was to compare 2 direct measurements for low-density lipoprotein cholesterol (LDL-C) with the Friedewald calculation (LDL-C [F]) in serum and their relationship with size-and charge-based LDL subfractions in serum ultracentrifugation fractions in patients with hypercholesterolemia (HC). <b><i>Methods and Results:</i></b> Serum samples from 283 HC patients who participated in a statin trial (the PATROL trial) were analyzed. Homogeneous assays for LDL-C were performed using reagents from Sekisui Medical (LDL-C [Se]) and Kyowa Medex (LDL-C [Ky]). Charge-based LDL subfractions were analyzed by capillary isotachophoresis (cITP). In whole serum in HC patients at baseline, LDL-C (Se) and LDL-C (Ky) negatively and positively deviated, respectively, from LDL-C (F). The negative deviation of LDL-C (Se) from LDL-C (F) increased with increasing LDL-C, while the positive deviation of LDL-C (Ky) from LDL-C (F) was positively correlated with charge-modified LDL (cmLDL) as analyzed by cITP. In serum d>1.006g/ml and >1.040g/ml fractions (LDL and small, dense LDL fractions, respectively), the deviation of LDL-C (Ky) from LDL-C (Se) was positively correlated with LDL-apoB (the number of LDL particles) and cmLDL. <b><i>Conclusions:</i></b> The 2 homogenous assays for LDL-C differed with regard to reactivity toward LDL particles and cmLDL in patients with HC. Direct measurement of LDL-C that reflects modified LDL, could be a better marker for the risk of coronary heart disease.  (<i>Circ J</i> 2012; <b>76:</b> 2241–2248)<br>

Journal

  • Circulation Journal

    Circulation Journal 76(9), 2241-2248, 2012-08-25

    The Japanese Circulation Society

References:  38

Cited by:  1

Codes

  • NII Article ID (NAID)
    10030697012
  • NII NACSIS-CAT ID (NCID)
    AA11591968
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13469843
  • Data Source
    CJP  CJPref  J-STAGE 
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