Genotoxicity and Cytotoxicity of Multi‐wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers
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- Asakura Masumi
- Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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- Sasaki Toshiaki
- Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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- Sugiyama Toshie
- Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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- Takaya Mitsutoshi
- National Institute of Occupational Safety and Health
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- Koda Shigeki
- National Institute of Occupational Safety and Health
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- Nagano Kasuke
- Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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- Arito Heihachiro
- Japan Bioassay Research Center, Japan Industrial Safety and Health Association
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- Fukushima Shoji
- Japan Bioassay Research Center, Japan Industrial Safety and Health Association
書誌事項
- タイトル別名
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- Genotoxicity and Cytotoxicity of Multi-wall Carbon Nanotubes in Cultured Chinese Hamster Lung Cells in Comparison with Chrysotile A Fibers
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Objectives: The potential applications and industrial production of multi-wall carbon nanotubes (MWCNT) have raised serious concerns about their safety for human health and the environment. The present study was designed to examine the in vitro cytotoxicity and genotoxicity of MWCNT and UICC chrysotile A (chrysotile). Methods: Cytotoxicity using both colony formation and lactate dehydrogenase (LDH) assays and genotoxicity including chromosome aberration, micronucleus induction and hgprt mutagenicity were examined by exposing cultured Chinese hamster lung (CHL/IU) cells to MWCNT or chrysotile at different concentrations. Results: The in vitro cytotoxicity of MWCNT depended on the solvent used for suspension of MWCNT and ultrasonication duration of the MWCNT suspension. A combination of DMSO/culture medium and 3-minute ultrasonication resulted in a well-dispersed medium with dispersion and isolation of agglomerated MWCNT by ultrasonication which manifested the highest cytotoxicity. The cytotoxicity was more potent for chrysotile than MWCNT. The genotoxicity of MWCNT was characterized by the formation of polyploidy without structural chromosome aberration, and an increased number of bi- and multi-nucleated cells without micronucleus induction, as well as negative hgprt mutagenicity. Chrysotile exhibited essentially the same genotoxicity as MWCNT, except for marginal but significant induction of micronuclei. MWCNT and chrysotile were incompletely internalized in the cells and localized in the cytoplasm. Conclusions: MWCNT and chrysotile were cytotoxic and genotoxic in Chinese hamster lung cells, but might interact indirectly with DNA. The results suggest that both test substances interfere physically with biological processes during cytokinesis.<br>
収録刊行物
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- journal of Occupational Health
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journal of Occupational Health 52 (3), 155-166, 2010
公益社団法人 日本産業衛生学会
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詳細情報 詳細情報について
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- CRID
- 1390282679430758144
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- NII論文ID
- 10030704971
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- NII書誌ID
- AA11090645
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- COI
- 1:CAS:528:DC%2BC3cXovFagtL0%3D
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- ISSN
- 13489585
- 13419145
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- NDL書誌ID
- 10696108
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- PubMed
- 20379079
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可