An Unusual Spliced Variant of DELLA Protein, a Negative Regulator of Gibberellin Signaling, in Lettuce

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  • SAWADA Yoshiaki
    Department of Bioresource Engineering, Yamagata University Program in the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University Department of Bioresource Engineering, Yamagata University Program in the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University
  • UMETSU Asami
    Department of Bioresource Engineering, Yamagata University Department of Bioresource Engineering, Yamagata University
  • KOMATSU Yuki
    Department of Bioresource Engineering, Yamagata University Department of Bioresource Engineering, Yamagata University
  • KITAMURA Jun
    Department of Applied Biological Chemistry, The University of Tokyo Department of Applied Biological Chemistry, The University of Tokyo
  • SUZUKI Hiroyuki
    Department of Applied Biological Chemistry, The University of Tokyo Department of Applied Biological Chemistry, The University of Tokyo
  • ASAMI Tadao
    Department of Applied Biological Chemistry, The University of Tokyo Department of Applied Biological Chemistry, The University of Tokyo
  • FUKUDA Machiko
    Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
  • HONDA Ichiro
    Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
  • MITSUHASHI Wataru
    Department of Bioresource Engineering, Yamagata University Program in the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University Department of Bioresource Engineering, Yamagata University Program in the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University
  • NAKAJIMA Masatoshi
    Department of Applied Biological Chemistry, The University of Tokyo Department of Applied Biological Chemistry, The University of Tokyo
  • TOYOMASU Tomonobu
    Department of Bioresource Engineering, Yamagata University Program in the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University Department of Bioresource Engineering, Yamagata University Program in the Science of Bioresource, The United Graduate School of Agricultural Science, Iwate University

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Abstract

DELLA proteins are negative regulators of the signaling of gibberellin (GA), a phytohormone regulating plant growth. DELLA degradation is triggered by its interaction with GID1, a soluble GA receptor, in the presence of bioactive GA. We isolated cDNA from a spliced variant of LsDELLA1 mRNA in lettuce, and named it LsDELLA1sv. It was deduced that LsDELLA1sv encodes truncated LsDELLA1, which has DELLA and VHYNP motifs at the N terminus but lacks part of the C-terminal GRAS domain. The recombinant LsDELLA1sv protein interacted with both Arabidopsis GID1 and lettuce GID1s in the presence of GA. A yeast two-hybrid assay suggested that LsDELLA1sv interacted with LsDELLA1. The ratio of LsDELLA1sv to LsDELLA1 transcripts was higher in flower samples at the late reproductive stage and seed samples (dry seeds and imbibed seeds) than in the other organ samples examined. This study suggests that LsDELLA1sv is a possible modulator of GA signaling in lettuce.

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