Separation and Identification of Rice Prolamins by Two-Dimensional Gel Electrophoresis and Amino Acid Sequencing

この論文にアクセスする

この論文をさがす

著者

    • SAITO Yuhi SAITO Yuhi
    • Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
    • SATOH Shigeru
    • Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
    • MASUMURA Takehiro
    • Laboratory of Genetic Engineering, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

抄録

There are difficulties in detecting and separating rice prolamin polypeptides by 2D-PAGE analysis because prolamin polypeptides are insoluble, and the amino acid sequences show high homology among them. In this study, we improved the prolamin extraction method and the 2D-PAGE procedure, and succeeded in separating prolamin polypeptide species by 2D-PAGE and in identifying major prolamin polypeptide sequences.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 76(3), 594-597, 2012-03-23

    公益社団法人 日本農芸化学会

参考文献:  20件中 1-20件 を表示

各種コード

  • NII論文ID(NAID)
    10030750991
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168451
  • NDL 記事登録ID
    023553920
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ