Overexpression of the (R)-Specific Enoyl-CoA Hydratase Gene from Pseudomonas chlororaphis HS21 in Pseudomonas Strains for the Biosynthesis of Polyhydroxyalkanoates of Altered Monomer Composition

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The (<I>R</I>)-specific enoyl-CoA hydratase gene (<I>phaJ</I><SUB>HS21</SUB>) from <I>Pseudomonas chlororaphis</I> HS21 was overexpressed in various <I>Pseudomonas</I> strains, alone and in combination with the polyhydroxyalkanoate synthase gene (<I>phaC</I><SUB>HS21</SUB>), for the biosynthesis of polyhydroxyalkanoates (PHAs) of altered monomer composition. Recombinant <I>Pseudomonas</I> strains harboring <I>phaC</I><SUB>HS21</SUB> and <I>phaJ</I><SUB>HS21</SUB> generated saturated and unsaturated monomers of C12–C14 in their PHAs. In particular, the level of the 3-hydroxytetradecenoate monomer in recombinant <I>P. chlororaphis</I> HS21 increased by approximately 260%. PhaJ<SUB>HS21</SUB> is expected to be useful in the biosynthesis of PHAs consisting of unusual monomer units.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 76(3), 613-616, 2012-03-23

    公益社団法人 日本農芸化学会

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各種コード

  • NII論文ID(NAID)
    10030751077
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09168451
  • NDL 記事登録ID
    023553958
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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