Anti-Aging Effects of Hesperidin on <I>Saccharomyces cerevisiae</I> <I>via</I> Inhibition of Reactive Oxygen Species and <I>UTH1</I> Gene Expression

• SUN Kaiyue

  College of Pharmaceutical Sciences, Zhejiang University College of Pharmaceutical Sciences, Zhejiang University

• XIANG Lan

  College of Pharmaceutical Sciences, Zhejiang University College of Pharmaceutical Sciences, Zhejiang University

• ISHIHARA Satoru

  Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University

• MATSUURA Akira

  Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University Department of Nanobiology, Graduate School of Advanced Integration Science, Chiba University

• SAKAGAMI Youji

  Graduate School of Bioagricultural Sciences, Nagoya University Graduate School of Bioagricultural Sciences, Nagoya University

• QI Jianhua

  College of Pharmaceutical Sciences, Zhejiang University College of Pharmaceutical Sciences, Zhejiang University

Bibliographic Information

Other Title

• Anti-Aging Effects of Hesperidin on Saccharomyces cerevisiae via Inhibition of Reactive Oxygen Species and UTH1 Gene Expression

Abstract

This study used a replicative lifespan assay of K6001 yeast to screen anti-aging food factors in commercial flavonoids. Hesperidin derived from the Citrus genus extended the lifespan of yeast at doses of 5 and 10 μM as compared with the control group (p<0.01, p<0.01). Reactive oxygen species (ROS), real-time PCR (RT-PCR), and lifespan assays of uth1 and skn7 mutants with the K6001 background were used to study the anti-aging mechanisms in yeast. The results indicate that hesperidin significantly inhibits the ROS of yeast, and UTH1 gene expression, and that SKN7 gene are involved in hesperidin-mediated lifespan extension. Further, increases in the Sir2 homolog, SIRT1 activity, and SOD gene expression were confirmed at doses of 5 (p<0.01) and 10 μM (p<0.05). This suggests that Sir2, UTH1 genes, and ROS inhibition after administration of hesperidin have important roles in the anti-aging effects of yeast. However, the aglycon hesperetin did not exhibit anti-aging effects in yeast.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (4), 640-645, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• hesperidin
• Sir2
• UTH1
• anti-aging
• reactive oxygen species (ROS)

Details

• CRID: 1390001206478603008
• NII Article ID: 10030751227
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110535
• COI: 1:STN:280:DC%2BC38rjt1SqsQ%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023592728
• PubMed: 22484922
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023592728
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110535
• Web Site: https://search.jamas.or.jp/link/ui/2012264967
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed