A Polyphenol Extract of <I>Hibiscus sabdariffa</I> L. Ameliorates Acetaminophen-Induced Hepatic Steatosis by Attenuating the Mitochondrial Dysfunction <I>in Vivo</I> and <I>in Vitro</I>

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  • LEE Chao-Hsin
    Division of Orthopaedics, Lee’s Medical Corporation Division of Orthopaedics, Lee’s Medical Corporation
  • KUO Chih-Yi
    Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Department of Clinical Laboratory, Tai-An Hospital Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Department of Clinical Laboratory, Tai-An Hospital
  • WANG Chau-Jong
    Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University
  • WANG Chi-Ping
    Department of Clinical Laboratory, Chung Shan Medical University Hospital Department of Clinical Laboratory, Chung Shan Medical University Hospital
  • LEE Yi-Ru
    Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Department of Pathology, Chung Shan Medical University Hospital Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Department of Pathology, Chung Shan Medical University Hospital
  • HUNG Chi-Nan
    Department of Holistic Wellness, Ming Dao University Department of Holistic Wellness, Ming Dao University
  • LEE Huei-Jane
    Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Department of Biochemistry, School of Medicine, Chung Shan Medical University Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University Department of Biochemistry, School of Medicine, Chung Shan Medical University

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  • A Polyphenol Extract of Hibiscus sabdariffa L. Ameliorates Acetaminophen-Induced Hepatic Steatosis by Attenuating the Mitochondrial Dysfunction in Vivo and in Vitro

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Abstract

Oxidative stress is the major contributor to acetaminophen (AAP)-caused liver damage. It promotes mitochondrial oxidative stress and collapses the mitochondrial membrane potential to cause cell death. We have previously shown that a polyphenol extract of Hibiscus sabdariffa L. (HPE) potentiated the antioxidative effect. We further examined in this study the possible mechanism of HPE against AAP-caused liver damage. BABL/c mice were orally fed with HPE (100, 200 or 300 mg/kg) for two weeks prior to an i.p. injection of 1000 mg/kg of AAP. The mice were decapitated 6 h after the AAP injection to collect the blood and liver for further determination. The results show that pretreating with HPE increased the level of glutathione (GSH), decreased the level of lipid peroxidation, and increased catalase activity in the liver. A histopathological evaluation shows that HPE could decrease AAP-induced liver sterosis accompanied by a decreased expression of AIF, Bax, Bid, and p-JNK in the liver. An in vitro assay revealed that HPE could reduce AAP-induced death of BABL/c normal liver cells (BNLs), reverse the lost mitochondrial potency and improve the antioxidative status, similarly to the results of the in vivo assay. We show in this study that HPE possessed the ability to protect the liver from AAP-caused injury. The protective mechanism might be regulated by decreasing oxidative stress and attenuating the mitochondrial dysfunction.

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