CUE Domain-Containing Protein Vps901 Is Required for Vacuolar Protein Transport in Schizosaccharomyces pombe

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The functions of two <I>Schizosaccharomyces pombe</I> Vps9-like genes, SPBC4F6.10/<I>vps901</I><SUP>+</SUP> and SPBC29A10.11c/<I>vps902</I><SUP>+</SUP>, were characterized. Genomic sequence analysis predicted that Vps901p contains a VPS9 domain, whereas cDNA analyses revealed that Vps901p contains a CUE domain (coupling of ubiquitin to ER degradation) in its C-terminal region. Deletion of <I>vps901</I><SUP>+</SUP> resulted in mis-sorting and secretion of <I>S. pombe</I> vacuolar carboxypeptidase Cpy1p, whereas deletion of <I>vps902</I><SUP>+</SUP> had no effect, suggesting that only Vps901p functions in vacuolar protein transport in <I>S. pombe</I>. Deletion of <I>vps901</I><SUP>+</SUP> further produced pleiotropic phenotypes, including vacuolar homotypic fusion and endocytosis defects. Heterologous expression of the budding yeast <I>VPS9</I> gene corrected the CPY mis-sorting defect in <I>vps901</I>Δ cells. These findings suggest that the VPS9 domain of Vps901p is required for vacuolar protein trafficking in <I>S. pombe</I>.

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  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 76(4), 652-659, 2012-04-23

    公益社団法人 日本農芸化学会

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各種コード

  • NII論文ID(NAID)
    10030751275
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    023592744
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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