Cloning, Monoclonal Antibody Production, and Bodily Distribution Pattern of a Bovine Lipocalin

  • JAPARIDZE Tamar
    Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
  • SENDA Akitsugu
    Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
  • NOZAKI Hirofumi
    Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology Research Center for Medical Glycoscience, National Institute of Advanced Industrial Science and Technology
  • YANAGIDA Mayumi
    Department of Agriculture and Life Science, Obihiro University of Agriculture and Veterinary Medicine Department of Agriculture and Life Science, Obihiro University of Agriculture and Veterinary Medicine
  • SUZUKI Takumi
    Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine
  • GANZORIG Khuukhenbaatar
    State Central Veterinary Laboratory State Central Veterinary Laboratory
  • KUSHI Yasunori
    Department of Materials and Applied Chemistry, Nihon University Department of Materials and Applied Chemistry, Nihon University
  • KIDA Katsuya
    Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine Field Center of Animal Science and Agriculture, Obihiro University of Agriculture and Veterinary Medicine
  • URASHIMA Tadasu
    Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine
  • BRUCKMAIER Rupert M.
    Veterinary Physiology, Vetsuisse Faculty, University of Bern Veterinary Physiology, Vetsuisse Faculty, University of Bern
  • FUKUDA Kenji
    Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine Department of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine

Search this article

Abstract

A bovine lipocalin, previously identified as a putative odorant-binding protein in bovine colostrum (bcOBP), was cloned and expressed, and its monoclonal antibody was established. bcOBP was constantly secreted into milk on day of parturition until at least 10 d postpartum at a concentration of 181±39 μg/L. Besides milk, bcOBP occurred in the nasal mucus, saliva, amniotic fluid, vaginal discharge, and blood plasma. Despite its low concentration, the distribution pattern and the finding that bcOBP harbored a characteristic sequence motif, CxxxC, which is conserved among insect and mammal pheromone binding proteins, suggest that bcOBP functions as a pheromone carrier. The presence of bcOBP in the plasma at varied concentrations depending on the lactation period does not exclude the possibility that bcOBP is secreted into milk from the blood. Cross-reactivity of the monoclonal antibody indicated presence of proteins homologous to bcOBP in the colostrum of farm animals of Cetartiodactyla.

Journal

References(61)*help

See more

Related Projects

See more

Details

Report a problem

Back to top