LGR4 Is Required for the Cell Survival of the Peripheral Mesenchyme at the Embryonic Stages of Nephrogenesis

• MOHRI Yasuaki

  Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University

• OYAMA Kazunori

  Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University

• SONE Mizuki

  Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University

• AKAMATSU Atsushi

  Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University

• NISHIMORI Katsuhiko

  Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University

Abstract

In mice, homozygous Lgr4 inactivation results in hypoplastic kidneys. To understand better the role of LGR4 in kidney development, we performed an analysis of kidneys in Lgr4^-/- embryos. We stained Lgr4^-/- kidneys with anti-WT1 and anti-Cleaved Caspase3 antibodies at E16.5, and observed that the structures of the cap mesenchyme were disrupted and that apoptosis increased. In addition, the expression of PAX2, an anti-apoptotic factor in kidney development, was also significantly decreased at E16.5. We found that the LGR4 defect caused an increase in apoptosis in the peripheral mesenchyme during kidney development.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (5), 888-891, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• LGR4
• knockout mice
• kidney morphogenesis
• apoptosis

Details

• CRID: 1390001206477653120
• NII Article ID: 10030752442
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110834
• COI: 1:STN:280:DC%2BC38jltlOhuw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023681825
• PubMed: 22738954
• Web Site: http://id.ndl.go.jp/bib/023681825
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023681825
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110834
• Web Site: https://search.jamas.or.jp/link/ui/2013092407
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed