Analysis of Two Gene Clusters Involved in 2,4,6-Trichlorophenol Degradation by Ralstonia pickettii DTP0602

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<i>Ralstonia pickettii</i> DTP0602 utilizes 2,4,6-trichlorophenol (2,4,6-TCP) as sole source of carbon and energy. We have characterized <i>hadABC</i> which is involved in the degradation of 2,4,6-TCP. To identify the other genes involved in 2,4,6-TCP degradation, the DNA sequence around <i>hadABC</i> was determined. A regulatory gene, <i>hadR</i>, homologous to the LysR-type transcriptional regulator was located upstream of <i>hadA</i>, but no maleylacetate (MA) reductase gene was located near <i>hadABC</i>. An 8.4-kb DNA fragment containing a MA reductase gene, <i>hadD</i>, was cloned using a DNA probe designed from the N-terminal sequence of purified MA reductase. <i>hadD</i> was located upstream of an open reading frame, <i>hadS</i>, which codes for a homolog of the LysR-type transcriptional regulator. A <i>hadS</i> insertion mutant, DTP62S, constitutively expressed MA reductase when grown on aspartate in the absence of 2,4,6-TCP. MA reductase was repressed in DTP62S supplemented with <i>hadS</i>. HadR and HadS are proposed to be a positive and a negative regulator, respectively. A draft genome sequence analysis revealed that the <i>hadRXABC</i> and <i>hadSYD</i> clusters were separated by 146-kb on the 8.1-Mb chromosome.

収録刊行物

  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 76(5), 892-899, 2012-05-23

    公益社団法人 日本農芸化学会

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各種コード

  • NII論文ID(NAID)
    10030752479
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    023681835
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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