Analysis of Two Gene Clusters Involved in 2,4,6-Trichlorophenol Degradation by <i>Ralstonia pickettii</i> DTP0602

• HATTA Takashi

  Department of Biomedical Engineering, Faculty of Engineering, Okayama University of Science Department of Biomedical Engineering, Faculty of Engineering, Okayama University of Science

• FUJII Eiji

  Industrial Technology Center of Okayama Prefecture Industrial Technology Center of Okayama Prefecture

• TAKIZAWA Noboru

  Department of Applied Chemistry, Faculty of Engineering, Okayama University of Science Department of Applied Chemistry, Faculty of Engineering, Okayama University of Science

Bibliographic Information

Other Title

• Analysis of Two Gene Clusters Involved in 2,4,6-Trichlorophenol Degradation by Ralstonia pickettii DTP0602

Abstract

Ralstonia pickettii DTP0602 utilizes 2,4,6-trichlorophenol (2,4,6-TCP) as sole source of carbon and energy. We have characterized hadABC which is involved in the degradation of 2,4,6-TCP. To identify the other genes involved in 2,4,6-TCP degradation, the DNA sequence around hadABC was determined. A regulatory gene, hadR, homologous to the LysR-type transcriptional regulator was located upstream of hadA, but no maleylacetate (MA) reductase gene was located near hadABC. An 8.4-kb DNA fragment containing a MA reductase gene, hadD, was cloned using a DNA probe designed from the N-terminal sequence of purified MA reductase. hadD was located upstream of an open reading frame, hadS, which codes for a homolog of the LysR-type transcriptional regulator. A hadS insertion mutant, DTP62S, constitutively expressed MA reductase when grown on aspartate in the absence of 2,4,6-TCP. MA reductase was repressed in DTP62S supplemented with hadS. HadR and HadS are proposed to be a positive and a negative regulator, respectively. A draft genome sequence analysis revealed that the hadRXABC and hadSYD clusters were separated by 146-kb on the 8.1-Mb chromosome.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (5), 892-899, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• 2,4,6-trichrolophenol
• chrolophenol
• <i>Ralstonia pickettii</i>

Details

• CRID: 1390282681454363264
• NII Article ID: 10030752479
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.110843
• COI: 1:STN:280:DC%2BC38jltlOgsg%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023681835
• PubMed: 22738955
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023681835
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110843
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed