Purification and Characterization of a 56kDa Chitinase Isozyme (PaChiB) from the Stomach of the Silver Croaker, Pennahia argentatus

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A 56 kDa chitinase isozyme (PaChiB) was purified from the stomach of the silver croaker <i>Pennahia argentatus</i>. The optimum pH and pH stability of PaChiB were observed in an acidic pH range. When <i>N</i>-acetylchitooligosaccharides ((GlcNAc)<sub>n</sub>, n=2 –6) were used as substrates, PaChiB degraded (GlcNAc)<sub>4 –6</sub> and produced (GlcNAc)<sub>2,3</sub>. It degraded (GlcNAc)<sub>5</sub> to produce (GlcNAc)<sub>2</sub> (23.2%) and (GlcNAc)<sub>3</sub> (76.8%). The ability to degrade <i>p</i>-nitrophenyl <i>N</i>-acetylchitooligosaccharides (pNp-(GlcNAc)<sub>n</sub>, n=2 –4) fell in the following order: pNp-(GlcNAc)<sub>3</sub> ≫ pNp-(GlcNAc)<sub>2</sub> > pNp-(GlcNAc)<sub>4</sub>. Based on these results, we concluded that PaChiB is an endo-type chitinolytic enzyme, and that it preferentially hydrolyzes the third glycosidic bond from the non-reducing end of (GlcNAc)<sub>n</sub>. Activity toward crystalline α- and β-chitin was activated at 124%–185% in the presence of 0.5 <small>M</small> NaCl. PaChiB exhibited markedly high substrate specificity toward crab-shell α-chitin.

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  • Bioscience, biotechnology, and biochemistry

    Bioscience, biotechnology, and biochemistry 76(5), 971-979, 2012-05-23

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10030752947
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    023681951
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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