An On-Demand Metalloprotease from Psychro-Tolerant Exiguobacterium undae Su-1, the Activity and Stability of Which Are Controlled by the Ca²⁺ Concentration

• HARADA Junko

  Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• TAKAKU Shusaku

  Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

• WATANABE Kunihiko

  Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University

書誌事項

タイトル別名

• An On-Demand Metalloprotease from Psychro-Tolerant <i>Exiguobacterium undae</i> Su-1, the Activity and Stability of Which Are Controlled by the Ca²⁺ Concentration

抄録

We reported an on-demand type of metalloprotease from Exiguobacterium undae Su-1. Although this species of bacterium is known to inhabit the permafrost, there are no reports on either strong proteases or peptidases. We found that Su-1 protease is superior to commercially available proteases in proteolytic activity in a lower to normal range of temperature (10–50 °C) as well as in rapid inactivation heat-dependently on the Ca²⁺ concentration. These characteristics meet well with the demands from food processing and manufacturing. Biochemical investigations of the purified enzyme and protein structural analysis after gene cloning confirmed that Su-1 protease conserved high identity in its primary sequence with thermophilic proteases of the M4 family. On the other hand, its flexibility was enhanced when one Ca²⁺ binding site was lost and by replacement for proline and isoleucine residues.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 76 (5), 986-992, 2012

  公益社団法人 日本農芸化学会

キーワード

• protease
• extracellular enzyme
• calcium ion
• psychrophile
• cold-adapted enzyme

詳細情報

• CRID: 1390001206478305152
• NII論文ID: 10030753012
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.110997
• COI: 1:STN:280:DC%2BC38jltlOntA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 023681973
• PubMed: 22738971
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023681973
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.110997
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可