Biosynthesis of Coumarin Glycosides by Transgenic Hairy Roots of Polygonum multiflorum

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  • ZHOU Liangbin
    Department of Natural Products Chemistry, Jinan University Department of Natural Products Chemistry, Jinan University
  • TIAN Tian
    Department of Pharmacology, Jinan University Department of Pharmacology, Jinan University
  • XUE Bailin
    Department of Natural Products Chemistry, Jinan University Department of Natural Products Chemistry, Jinan University
  • SONG Liyan
    Department of Pharmacology, Jinan University Department of Pharmacology, Jinan University
  • LIU Ling
    Department of Natural Products Chemistry, Jinan University Department of Natural Products Chemistry, Jinan University
  • YU Rongmin
    Department of Natural Products Chemistry, Jinan University Department of Natural Products Chemistry, Jinan University

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  • Biosynthesis of Coumarin Glycosides by Transgenic Hairy Roots of <i>Polygonum multiflorum</i>

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To investigate the substrate specificity and regio-selectivity of coumarin glycosyltransferases in transgenic hairy roots of Polygonum multiflorum, esculetin (1) and eight hydroxycoumarins (2–9) were employed as substrates. Nine corresponding glycosides (10–18) involving four new compounds, 6-chloro-4-methylcoumarin 7-O-β-D-glucopyranoside (15), 6-chloro-4-phenylcoumarin 7-O-β-D-glucopyranoside (16), 8-hydroxy-4-methylcoumarin 7-O-β-D-glucopyranoside (17), and 8-allyl-4-methylcoumarin 7-O-β-D-glucopyranoside (18), were biosynthesized by the hairy roots.

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