Select Nutrients in the Uterine Lumen of Sheep and Pigs Affect Conceptus Development

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Interferon tau (IFNT) is the pregnancy recognition signal from ruminant conceptuses. IFNT also acts with P4 to induce expression of genes for transport of nutrients, such as glucose (Gluc) and arginine (Arg) into the uterine lumen to activate mechanistic mammalian target of rapamycin (MTOR) cell signaling that stimulates proliferation, migration, gene transcription and mRNA translation by conceptus trophectoderm (Tr). In ewes, Arg and Gluc increase significantly in the uterine lumen between Days 10 and 15 of pregnancy due to increased expression of transporters for Gluc (<i>SLC2A1</i> and <i>SLC5A1</i>) and Arg (S<i>LC7A2B</i>) by uterine epithelia. Arg and Gluc stimulate proliferation, migration and mRNA translation by Tr. Arg increases expression of GTP cyclohydrolase 1 (<i>GCH1</i>) and <i>IFNT</i> mRNAs while Arg and Gluc increase ornithine decarboxylase, nitric oxide synthase 2, and <i>GCH1</i> mRNAs and proteins by Tr cells. GCH1 is required for synthesis of tetrahydrobiopterin, an essential cofactor for all NOS isoforms. Arg is metabolized to nitric oxide and polyamines that increase proliferation and migration of Tr cells. In pigs, Gluc, Arg, leucine (Leu) and glutamine (Gln) increase in the uterine lumen between Days 12 and 15 of pregnancy due to enhanced expression of transporters for Gluc and amino acids. Transporters for Gluc in porcine uterine LE (<i>SLC2A1</i>) and conceptus trophectoderm (<i>SLC2A2</i>) are abundant. Transporters for glutamate and neutral (<i>SLC1A1</i>, <i>SLC1A4</i>) and cationic (S<i>LC7A1</i>, <i>SLC7A2</i>, <i>SLC7A7</i>, <i>SLC7A9</i>) amino acids are expressed in uterine LE and <i>SLC7A3</i> mRNA is expressed in conceptus Tr. Arg and Leu increase MTOR cell signaling and proliferation of pig Tr, as do Gluc and fructose. Azaserine, an inhibitor of hexosamine biosynthesis, inhibits effects of Gluc and fructose. Thus, select nutrients in the uterine lumen affect gene transcription and mRNA translation to affect conceptus development.

収録刊行物

  • The Journal of reproduction and development

    The Journal of reproduction and development 58(2), 180-188, 2012-04-01

    日本繁殖生物学会

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各種コード

  • NII論文ID(NAID)
    10030753562
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    REV
  • ISSN
    09168818
  • NDL 記事登録ID
    023601990
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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