Season-Induced Changes in Bovine Sperm Motility Following a Freeze-Thaw Procedure

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著者

    • BIRAN David
    • The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University
    • FRIEDMAN Eran
    • The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University
    • DRUKER Shaked
    • The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University
    • ROTH Zvi
    • The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University

抄録

Decreased conception rate of dairy cows in the summer is mainly associated with the deleterious effects of environmental thermal stress on the female reproductive tract. Here, we suggest that decreased reproductive performance might be partially due to inferior-quality semen. Semen from five representative bulls was collected in summer (August to September) and winter (December to January) and evaluated with a computerized sperm-quality analyzer for bulls (SQA-Vb). No seasonal effect was found in fresh ejaculate, but sperm examined post-thawing showed lower velocity, motility and progressive motility (P<0.04) in summer <i>vs.</i> winter samples. Element concentrations in the seminal plasma, determined by inductively coupled plasma-atomic emission spectrometry, differed between seasons, with higher (P<0.01) concentration values of K, Mg, Na and S elements in winter <i>vs.</i> summer samples. Therefore, season-induced alterations in seminal plasma element concentration should be taken into account when using an extender for cryopreservation. Acrosome integrity was assessed by a triple-fluorescence test using Hoechst 33342, fluorescein isothiocyanate-conjugated <i>Pisum sativum</i> agglutinin (FITC-PSA) and propidium iodide. Acrosome reaction was examined by a one-step staining method using FITC-PSA. The proportion of sperm cells with a damaged acrosome post-thawing tended to be higher (P<0.07) in semen collected during the summer <i>vs.</i> winter. Such alterations suggest that seasonal reductions in sperm function might also be involved in the decreased conception rate of dairy cows in summer.<br>

収録刊行物

  • The Journal of reproduction and development

    The Journal of reproduction and development 58(2), 212-218, 2012-04-01

    日本繁殖生物学会

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各種コード

  • NII論文ID(NAID)
    10030753852
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    023602036
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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