GATA4 is Involved in the Gonadal Development and Maturation of the Teleost Fish Tilapia, Oreochromis niloticus

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著者

    • LI Jianzhong LI Jianzhong
    • Key Lab of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University
    • CHEN Wenjuan CHEN Wenjuan
    • Key Lab of Protein Chemistry and Developmental Biology of the Ministry of Education, College of Life Sciences, Hunan Normal University
    • ZHOU Linyan
    • Laboratory of Reproductive Biology, National Institute for Basic Biology
    • SAKAI Fumie
    • Laboratory of Reproductive Biology, National Institute for Basic Biology
    • GUAN Guijun
    • Laboratory of Reproductive Biology, National Institute for Basic Biology

抄録

GATA4, a member of the GATA family, is a well-known transcription factor implicated in the regulation of sex determination and sexual differentiation in mammals. However, little is known about the possible role of GATA4 in fish reproduction. In the present study, a full-length <i>GATA4</i> cDNA from the tilapia was cloned and characterized. The tilapia <i>GATA4</i> gene contained an open reading frame (ORF) of 1179 nucleotides encoding a protein of 392 amino acids. Sequence alignment revealed that the tilapia GATA4 protein shared higher homology (ranging from 63.1 to 74.6%) with other vertebrates. RT-PCR analysis indicated that the <i>GATA4</i> gene is expressed in the ovary, testis, liver, intestine and heart in adult tilapia. <i>In situ</i> hybridization was performed to examine the temporal and spatial expression patterns of <i>GATA4</i> during tilapia gonadal differentiation and development. In the undifferentiated gonad, <i>GATA4</i> was expressed in the somatic cells of both sexes. Subsequently, <i>GATA4</i> expression persisted in the differentiated, juvenile and adult ovary and testis in tilapia. Our data indicate for the first time that <i>GATA4</i> is not only necessary for the onset of gonadal differentiation, but also important for gonadal development and maturation.

収録刊行物

  • The Journal of reproduction and development

    The Journal of reproduction and development 58(2), 237-242, 2012-04-01

    日本繁殖生物学会

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各種コード

  • NII論文ID(NAID)
    10030754005
  • NII書誌ID(NCID)
    AA10936678
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168818
  • NDL 記事登録ID
    023602097
  • NDL 請求記号
    Z54-H305
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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