Synthesis and Characterization of TiO₂ Nanoparticles: Applications in Research on the Interaction of Colloidal TiO₂ with Human Serum Albumin by Fluorescence Spectroscopy Synthesis and Characterization of TiO_2 Nanoparticles : Applications in Research on the Interaction of Colloidal TiO_2 with Human Serum Albumin by Fluorescence Spectroscopy

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Titanium dioxide (TiO<sub>2</sub>) nanoparticles (NPs) are widely used as an important kind of biomaterials due to their large surface area, enhanced chemical reactivity and easy penetration into cells. Nano TiO<sub>2</sub> with pure anatase phase was successfully prepared by solvothermal method. Its particle size was about 21 nm, while the larger specific surface area of TiO<sub>2</sub> was 77.43 m<sup>2</sup>/g. The interaction of colloidal TiO<sub>2</sub> with human serum albumin was studied by using absorption spectra and fluorescence spectra. The apparent binding constants (<i>K</i>) were 345.780 × 10<sup>5</sup>, 4.376 × 10<sup>5</sup>, 0.035 × 10<sup>5</sup> at 298, 303 and 308 K, respectively. In addition, the number of binding sites (<i>n</i>) was gradually decreased with the increase of temperature, which indicated that the quenching mechanism of albumin by colloidal TiO<sub>2</sub> was static fluorescence quenching process. Based on fluorescence resonance energy transfer, the energy transfer efficiency (<i>E</i>) and critical transfer distance (<i>r</i><sub>0</sub>) between donor (human serum albumin, HSA) and acceptor (colloidal TiO<sub>2</sub>) were calculated to be 0.862 and 6.244 nm, which suggested that non-radiative energy transfer occurred between TiO<sub>2</sub> and HSA. Furthermore, the conformational changes of HSA were shown by synchronous fluorescence.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 28(5), 491-496, 2012-05-10

    The Japan Society for Analytical Chemistry

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各種コード

  • NII論文ID(NAID)
    10030757562
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09106340
  • NDL 記事登録ID
    023613997
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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