Photochemical Immobilization of Cells onto a Glass Substrate for in situ DNA Analysis

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著者

    • ISU Anri ISU Anri
    • Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
    • SATO Kae
    • Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

抄録

A simple and robust method to immobilize cells onto a glass substrate is presented. The method employs a photochemical reaction of benzophenone, which is modified on the substrate using a standard silane coupling agent, with cells. Cells were immobilized to an area irradiated with UV light from a standard light source under an inverted microscope. The dependence of immobilization on the light power intensity and irradiation time was investigated. <i>In situ</i> DNA analysis within the immobilized cells was demonstrated using target-primed rolling circle amplification and fluorescent detection.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 28(6), 537-539, 2012-06-10

    The Japan Society for Analytical Chemistry

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各種コード

  • NII論文ID(NAID)
    10030757784
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    09106340
  • NDL 記事登録ID
    023689163
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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