Elution Behavior of Lambda-DNA with Ternary Mixed Carrier Solvents in an Open-Tubular Capillary under Laminar Flow Conditions

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著者

    • HASHIMOTO Masahiko
    • Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University
    • TSUKAGOSHI Kazuhiko
    • Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

抄録

An open-tubular capillary chromatography was developed based on the tube radial distribution of the ternary mixed carrier solvents that generated the inner and outer phases under laminar flow conditions. This is called “tube radial distribution chromatography” (TRDC). In this report, the elution behavior of lambda-DNA (48502 bp) as a biopolymer was examined by the TRDC system. The ternary mixture of water–acetonitrile–ethyl acetate, 15:3:2 or 3:8:4 volume ratio, as a carrier solution was fed into the capillary tube made of polytetrafluoroethylene (PTFE) or fused-silica. The mixture of hydrophobic 1-naphthol and hydrophilic lambda-DNA was subjected to the TRDC system using the water-rich carrier solution. Lambda-DNA and 1-naphthol were distributed between the inner and outer phases due to their hydrophilic and hydrophobic nature, and then eluted in this order, undergoing chromatographic separation. The mixture of hydrophilic 2,6-naphthalenedisulfonic acid and hydrophobic lambda-DNA that was treated with surfactants was also examined with the organic solvent-rich carrier solution. The modified hydrophobic DNA and 2,6-naphthalenedisulfonic acid were distributed and eluted in this order due to their nature.

収録刊行物

  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

    Analytical sciences : the international journal of the Japan Society for Analytical Chemistry 28(6), 617-620, 2012-06-10

    社団法人 日本分析化学会

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各種コード

  • NII論文ID(NAID)
    10030758173
  • NII書誌ID(NCID)
    AA10500785
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    09106340
  • NDL 記事登録ID
    023689233
  • NDL 請求記号
    Z54-F482
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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