Elution Behavior of Lambda-DNA with Ternary Mixed Carrier Solvents in an Open-Tubular Capillary under Laminar Flow Conditions

• NOGAMI Takahiro

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• FUJINAGA Satoshi

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• JINNO Naoya

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• HASHIMOTO Masahiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

• TSUKAGOSHI Kazuhiko

  Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University

Abstract

An open-tubular capillary chromatography was developed based on the tube radial distribution of the ternary mixed carrier solvents that generated the inner and outer phases under laminar flow conditions. This is called “tube radial distribution chromatography” (TRDC). In this report, the elution behavior of lambda-DNA (48502 bp) as a biopolymer was examined by the TRDC system. The ternary mixture of water–acetonitrile–ethyl acetate, 15:3:2 or 3:8:4 volume ratio, as a carrier solution was fed into the capillary tube made of polytetrafluoroethylene (PTFE) or fused-silica. The mixture of hydrophobic 1-naphthol and hydrophilic lambda-DNA was subjected to the TRDC system using the water-rich carrier solution. Lambda-DNA and 1-naphthol were distributed between the inner and outer phases due to their hydrophilic and hydrophobic nature, and then eluted in this order, undergoing chromatographic separation. The mixture of hydrophilic 2,6-naphthalenedisulfonic acid and hydrophobic lambda-DNA that was treated with surfactants was also examined with the organic solvent-rich carrier solution. The modified hydrophobic DNA and 2,6-naphthalenedisulfonic acid were distributed and eluted in this order due to their nature.

Journal

• Analytical Sciences

  Analytical Sciences 28 (6), 617-620, 2012

  The Japan Society for Analytical Chemistry

Keywords

• Analytical Chemistry

Details

• CRID: 1390001204259743360
• NII Article ID: 10030758173
• NII Book ID: AA10500785
• DOI: 10.2116/analsci.28.617
• COI: 1:STN:280:DC%2BC38jksVKisw%3D%3D
• ISSN: 13482246
• ISSN: 09106340
• NDL BIB ID: 023689233
• PubMed: 22729050
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023689233
• Web Site: https://link.springer.com/content/pdf/10.2116/analsci.28.617.pdf
• Web Site: https://link.springer.com/article/10.2116/analsci.28.617/fulltext.html
• Web Site: https://www.jstage.jst.go.jp/article/analsci/28/6/28_617/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed