Fibulin-5 Protein Is Reduced in the Lung of Patients with Spontaneous Pneumothorax Who Are Under 25 Years Old

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Abstract

Purpose: To clarify whether fibulins-5 is associated with primary spontaneous pneumothorax (PSP) in young PSP patients. <br>Methods: Forty-six surgically resected, fresh lung specimens were used. Patients were divided into 3 groups: younger than 25 years with pneumothorax (group Y), 25 years or older with pneumothorax (group O), and without pneumothorax (group C). Chest X-ray, computed tomography data, height/width ratio (H/W) and anteroposterior/transverse diameter ratio (a/b) were measured. Elastica van Gieson staining and immunofluorescence staining for fibulin-5 were performed. Fibulin-5 mRNA expression and protein levels were measured by real-time PCR and western blotting. Direct sequences of the fibulin-5 gene in PSP patients were performed.<br>Results: The mean H/W ratio in group Y was significantly larger than that in the other groups (p <0.01). The mean a/b ratio in group Y was significantly smaller than that in the other groups (p = 0.02). Fibulin-5 mRNA expression was not significantly different among the groups (p = 0.64). The relative intensity of fibulin-5 protein in group Y was significantly lower than that in group O (p = 0.006), with no significant differences between groups O and C (p = 0.14).<br>Conclusions: We showed that fibulin-5 is reduced in patients with PSP who are younger than 25 years.

Journal

  • Annals of Thoracic and Cardiovascular Surgery

    Annals of Thoracic and Cardiovascular Surgery 18(3), 200-205, 2012-06-01

    The Editorial Committee of Annals of Thoracic and Cardiovascular Surgery

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