Identification of the glutathione S-transferase gene responsible for flower color intensity in carnations

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Abstract

Two cDNAs with homology to <i>glutathione</i> S-<i>transferase</i> (<i>GST</i>) were isolated from the carnation (<i>Dianthus caryophyllus</i>); these cDNAs are termed here <i>DcGSTF1</i> and <i>DcGSTF2</i>. Phylogenetic analysis suggested that both DcGSTF1 and DcGSTF2 belonged to the Phi class of GSTs. <i>DcGSTF2</i> showed high levels of transcription at late stages of petal development when anthocyanin biosynthesis is most active. Sequencing of <i>DcGSTF2</i> indicated that it consisted of three exons and two introns. A truncated <i>DcGSTF2</i> gene, resulting from the insertion of a CACTA-type transposable element, was found in the genome of a mutable flower line bearing deep pink sectors on pale pink petals. A full length <i>DcGSTF2</i> gene driven by a continuous expression promoter was introduced into the epidermal cells of carnations with pale pink petals. The transformed cells were deep pink. These results suggest that the <i>DcGSTF2</i> gene is responsible for flower color intensity in carnations.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29(3), 223-227, 2012-06-25

    Japanese Society for Plant Cell and Molecular Biology

References:  17

Cited by:  2

Codes

  • NII Article ID (NAID)
    10030804543
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    023784235
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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