Accumulation of antioxidants and antioxidant activity in tomato, Solanum lycopersicum, are enhanced by the transcription factor SlICE1

Access this Article

Search this Article

Author(s)

Abstract

Antioxidants and antioxidant activity confer important protective effects in plants against the effects of free radicals, which are generated by biotic and abiotic stresses, such as cold. In another study, we identified <i>SlICE1</i> as a basic helix–loop–helix transcription factor to improve cold tolerance. Here, we demonstrate that <i>SlICE1</i> plays an important role in the accumulation of antioxidants and in the regulation of antioxidant activity in tomato <i>Solanum lycopersicum</i>. Overexpression of <i>SlICE1</i> in tomatoes enhanced the accumulation of antioxidants, such as β-carotene, lycopene, and ascorbic acid, as well as antioxidant activity, measured as the scavenging of 2,2-diphenyl-1-picrylhydazyl (DPPH) free radicals and O<sub>2</sub><sup>-</sup> radicals. Furthermore, the sugar content in <i>SlICE1</i>-overexpressing tomatoes red fruits was higher than that in wild-type red fruits. Metabolite profiling analysis performed by capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS) revealed that several amino acids and amines were more highly accumulated in <i>SlICE1</i>-overexpressing tomato red fruits compared to those in wild-type tomatoes. These results suggest that <i>SlICE1</i> plays a role in the regulation of antioxidant activity through the accumulation of several antioxidants.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29(3), 261-269, 2012-06-25

    Japanese Society for Plant Cell and Molecular Biology

References:  51

Cited by:  2

Codes

  • NII Article ID (NAID)
    10030804692
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13424580
  • NDL Article ID
    023784412
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top