Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an Agrobacterium-mediated RNAi technique

DOI DOI Web Site 被引用文献2件 参考文献21件
  • Miyawaki Katsuyuki
    Department of Life Systems, Institute of Technology and Science, The University of Tokushima
  • Fukuoka Sachi
    Department of Life Systems, Institute of Technology and Science, The University of Tokushima
  • Kadomura Yasuko
    Department of Nutrition, Faculty of Medicine, The University of Tokushima
  • Hamaoka Hirokazu
    Department of Life Systems, Institute of Technology and Science, The University of Tokushima
  • Mito Taro
    Department of Life Systems, Institute of Technology and Science, The University of Tokushima
  • Ohuchi Hideyo
    Department of Life Systems, Institute of Technology and Science, The University of Tokushima
  • Schwab Wilfried
    Biotechnology of Natural Products, Technical University München
  • Noji Sumihare
    Department of Life Systems, Institute of Technology and Science, The University of Tokushima

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タイトル別名
  • Establishment of a novel system to elucidate the mechanisms underlying light-induced ripening of strawberry fruit with an <i>Agrobacterium</i>-mediated RNAi technique
  • 10.5511/plantbiotechnology.12.406a

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抄録

Traditional methods used to study strawberry ripening-related gene function are time-consuming, and require at least 15 months from initiating the transformation experiment until the first ripe fruits are available for analysis. To accelerate data acquisition during gene function studies, we explored a transient assay method that employs an Agrobacterium-mediated RNAi (AmRNAi) technique in post-harvest strawberry fruit, Fragaria×ananassa (Fa) cv. Sachinoka, a Japanese cultivar. Our results showed that artificial white light induced strong expression of Fa′chalcone synthase (Fa′CHS), Fa′chalcone isomerase (Fa′CHI), and Fa′flavonoid 3′-hydroxylase orthologues (Fa′F3′H) in post-harvest fruit. Fa′CHS and Fa′F3′H function was subsequently examined by performing AmRNAi with post-harvest fruit. Although reduction of light-induced Fa′F3′H expression by AmRNAi resulted in no significant change in anthocyanin content, reduction of Fa′CHS significantly decreased anthocyanin levels, and up-regulated Fa′F3′H levels. Our results are consistent with previous data indicating that while CHS is required for anthocyanin accumulation during late stage strawberry fruit maturation, Fa′F3′H is not required. The novel system described here enabled gene function data to be available within 10 days of initiating the incubation period following infiltration. Therefore, we conclude our system is a valuable tool to elucidate the molecular mechanisms underlying light-induced ripening of strawberry fruit.

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