Sugar-inducible RPT2a, a subunit of 26S proteasome, participates in sugar response in Arabidopsis

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Abstract

The ubiquitin/26S proteasome system (UPS) plays a central role in the degradation of short-lived regulatory proteins that control many cellular events. In this study, the <i>Arabidopsis</i> knockout mutant <i>rpt2a</i>, which contains a defect in the AtRPT2a subunit of the 26S proteasome regulatory particle, showed hypersensitivity to sugars as well as enlarged leaves. When the role of RPT2a in sugar response was examined in further detail it was found that putatively only the <i>AtRPT2a</i> gene of 19S proteasome was markedly transcriptionally promoted by sugar application. Notably, poly-ubiquitinated proteins degraded by the UPS accumulated significantly in <i>rpt2a</i> mutant under 6% sucrose conditions compared to wild type. In addition, the <i>AtRPT2a</i> gene in <i>gin2</i>, a glucose insensitive mutant with a defective glucose-sensing hexokinase, was not upregulated by sugar application, indicating that AtRPT2a is involved in hexokinase-dependent sugar response. Taken together, the above findings indicate that AtRPT2a plays an essential role in the maintenance of proteasome-dependent proteolysis activity in response to sugars.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29(3), 279-284, 2012-06-25

    Japanese Society for Plant Cell and Molecular Biology

References:  26

Codes

  • NII Article ID (NAID)
    10030804760
  • NII NACSIS-CAT ID (NCID)
    AA11250821
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13424580
  • NDL Article ID
    023784478
  • NDL Call No.
    Z54-J126
  • Data Source
    CJP  NDL  J-STAGE 
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