Important Role of β₁-Integrin in Fucoidan-Induced Apoptosis <i>via</i> Caspase-8 Activation

• YAMASAKI Yumi

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University Department of Biochemistry and Applied BioScience, Faculty of Agriculture, University of Miyazaki Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University Department of Biochemistry and Applied BioScience, Faculty of Agriculture, University of Miyazaki

• YAMASAKI Masao

  Department of Biochemistry and Applied BioScience, Faculty of Agriculture, University of Miyazaki Department of Biochemistry and Applied BioScience, Faculty of Agriculture, University of Miyazaki

• TACHIBANA Hirofumi

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

• YAMADA Koji

  Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University

Bibliographic Information

Other Title

• Important Role of β₁-Integrin in Fucoidan-Induced Apoptosis via Caspase-8 Activation
• Important role of beta1-integrin in fucoidan-induced apoptosis via caspase-8 activation

Abstract

Fucoidan induces apoptosis by activating caspase-8 in human MCF-7 breast cancer cells, but the detailed mechanism for this is not understood. We demonstrate here that fucoidan interacted with the cell surface, and silencing the β₁-integrin gene expression inhibited fucoidan-induced apoptosis accompanied by caspase-8 activation. Fucoidan induced formation of the β₁-integrin-caspase-8 complex. These data indicate that β₁-integrin is an important factor for the cell-surface binding of fucoidan and plays an important role in fucoidan-induced apoptosis. Fucoidan also induced recruitment of caspase-8 to the β₁-integrin intracellular domain, cleaved it into the activated protein by direct combination with β₁-integrin, and induced apoptosis via the caspase cascade in MCF-7 cells.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 76 (6), 1163-1168, 2012

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• fucoidan
• anoikis
• apoptosis
• integrin
• caspase-8

Details

• CRID: 1390282681454771840
• NII Article ID: 10030818193
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.111001
• COI: 1:STN:280:DC%2BC38jps1Ghuw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 023768375
• PubMed: 22790940
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023768375
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed