Novel Conjugative Transferable Multiple Drug Resistance Plasmid pAQU1 from <i>Photobacterium damselae</i> subsp. <i>damselae</i> Isolated from Marine Aquaculture Environment

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  • Nonaka Lisa
    Department of Microbiology, Dokkyo Medical University School of Medicine Department of Microbiology, Dokkyo Medical University School of Medicine
  • Maruyama Fumito
    Section of Bacterial Pathogenesis, Section of Microbial Genomics and Ecology, Tokyo Medical and Dental University, Graduate School of Medical and Dental Science Section of Bacterial Pathogenesis, Section of Microbial Genomics and Ecology, Tokyo Medical and Dental University, Graduate School of Medical and Dental Science
  • Miyamoto Manabu
    Department of Microbiology, Dokkyo Medical University School of Medicine Department of Microbiology, Dokkyo Medical University School of Medicine
  • Miyakoshi Masatoshi
    Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University
  • Kurokawa Ken
    Department of Biological Information, Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology Department of Biological Information, Tokyo Institute of Technology, Graduate School of Bioscience and Biotechnology
  • Masuda Michiaki
    Department of Microbiology, Dokkyo Medical University School of Medicine Department of Microbiology, Dokkyo Medical University School of Medicine

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Other Title
  • Novel Conjugative Transferable Multiple Drug Resistance Plasmid pAQU1 from Photobacterium damselae subsp. damselae Isolated from Marine Aquaculture Environment
  • Novel conjugative transferable multiple drug resistance plasmid pAQU1 from <italic>Photobacterium damselae</italic> subsp. <italic>damselae</italic> isolated from marine aquaculture environment
  • Novel conjugative transferable multidrug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment.

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Abstract

The emergence of drug-resistant bacteria is a severe problem in aquaculture. The ability of drug resistance genes to transfer from a bacterial cell to another is thought to be responsible for the wide dissemination of these genes in the aquaculture environment; however, little is known about the gene transfer mechanisms in marine bacteria. In this study, we show that a tetracycline-resistant strain of Photobacterium damselae subsp. damselae, isolated from seawater at a coastal aquaculture site in Japan, harbors a novel multiple drug resistance plasmid. This plasmid named pAQU1 can be transferred to Escherichia coli by conjugation. Nucleotide sequencing showed that the plasmid was 204,052 base pairs and contained 235 predicted coding sequences. Annotation showed that pAQU1 did not have known repA, suggesting a new replicon, and contained seven drug resistance genes: blaCARB-9-like, floR, mph(A)-like, mef(A)-like, sul2, tet(M) and tet(B). The plasmid has a complete set of genes encoding the apparatus for the type IV secretion system with a unique duplication of traA. Phylogenetic analysis of the deduced amino acid sequence of relaxase encoded by traI in pAQU1 demonstrated that the conjugative transfer system of the plasmid belongs to MOBH12, a sub-group of the MOBH plasmid family, closely related to the IncA/C type of plasmids and SXT/R391 widely distributed among species of Enterobacteriaceae and Vibrionaceae. Our data suggest that conjugative transfer is involved in horizontal gene transfer among marine bacteria and provide useful insights into the molecular basis for the dissemination of drug resistance genes among bacteria in the aquaculture environment.<br>

Journal

  • Microbes and Environments

    Microbes and Environments 27 (3), 263-272, 2012

    Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

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