Rapid Enumeration of Active <i>Legionella pneumophila</i> in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining

• Baba Takashi

  Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University

• Inoue Naoko

  Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University

• Yamaguchi Nobuyasu

  Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University

• Nasu Masao

  Graduate School of Pharmaceutical Sciences, Osaka University Graduate School of Pharmaceutical Sciences, Osaka University

Bibliographic Information

Other Title

• Rapid Enumeration of Active Legionella pneumophila in Freshwater Environments by the Microcolony Method Combined with Direct Fluorescent Antibody Staining

Abstract

In this study, a microcolony technique was combined with direct fluorescent antibody staining for the specific detection and enumeration of Legionella pneumophila in freshwater samples with growth activity. This method allowed the detection of active L. pneumophila (within 48 h) in 91 bath water samples collected from 30 bathing facilities, with similar sensitivity of a conventional plate-counting method. These results suggest that the microcolony method combined with fluorescent antibody staining could be useful as a monitoring technique for the prevention of Legionnaires’ disease through the early detection of L. pneumophila in freshwater.<br>

Journal

• Microbes and Environments

  Microbes and Environments 27 (3), 324-326, 2012

  Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles

Keywords

• <i>Legionella pneumophila</i>
• rapid enumeration
• microcolony
• growth activity
• freshwater

Details

• CRID: 1390282679323218176
• NII Article ID: 10030869731
• NII Book ID: AA11551577
• DOI: 10.1264/jsme2.me11324
• COI: 1:STN:280:DC%2BC38vptlGmuw%3D%3D
• ISSN: 13474405
• ISSN: 13426311
• NDL BIB ID: 023919785
• PubMed: 22446304
• Web Site: http://id.ndl.go.jp/bib/023919785
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023919785
• Web Site: https://www.jstage.jst.go.jp/article/jsme2/27/3/27_ME11324/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed