Technological Advances in Bifidobacterial Molecular Genetics: Application to Functional Genomics and Medical Treatments

• FUKIYA Satoru

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• HIRAYAMA Yosuke

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• SAKANAKA Mikiyasu

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

• KANO Yasunobu

  Department of Molecular Genetics, Kyoto Pharmaceutical University

• YOKOTA Atsushi

  Laboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University

抄録

Bifidobacteria are well known as beneficial intestinal bacteria that exert health-promoting effects in humans. In addition to physiological and immunological investigations, molecular genetic technologies have been developed and have recently started to be applied to clarify the molecular bases of host-Bifidobacterium interactions. These technologies include transformation technologies and Escherichia coli-Bifidobacterium shuttle vectors that enable heterologous gene expression. In this context, a plasmid artificial modification method that protects the introduced plasmid from the restriction system in host bifidobacteria has recently been developed to increase transformation efficiency. On the other hand, targeted gene inactivation systems, which are vital for functional genomics, seemed far from being practically applicable in bifidobacteria. However, remarkable progress in this technology has recently been achieved, enabling functional genomics in bifidobacteria. Integrated use of these molecular genetic technologies with omics-based analyses will surely boost characterization of the molecular basis underlying beneficial effects of bifidobacteria. Applications of recombinant bifidobacteria to medical treatments have also progressed.<br>

収録刊行物

• Bioscience of Microbiota, Food and Health

  Bioscience of Microbiota, Food and Health 31 (2), 15-25, 2012

  BMFH出版会

キーワード

• <i>Bifidobacterium</i>
• molecular genetics
• cryptic plasmid
• heterologous gene expression
• targeted gene inactivation
• functional genomics
• enzyme/prodrug therapy

詳細情報

• CRID: 1390001205410870784
• NII論文ID: 130004562662
• NII論文ID: 10030872664
• NII書誌ID: AA12588288
• DOI: 10.12938/bmfh.31.15
• ISSN: 21863342
• Web Site: https://www.jstage.jst.go.jp/article/bmfh/31/2/31_2_15/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013046792
• 本文言語コード: en
• データソース種別:
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• 抄録ライセンスフラグ: 使用不可