A novel glass ionomer cement containing MgCO_3 apatite induced the increased proliferation and differentiation of human pulp cells in vitro

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Author(s)

    • LAITEERAPONG Arunee
    • Dental Biomaterials Science, Graduate School-Interdisciplinary Program, Chulalongkorn University
    • LOCHAIWATANA Yossakit
    • Dental Biomaterials Science, Graduate School-Interdisciplinary Program, Chulalongkorn University
    • HIRATA Isao
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
    • OKAZAKI Masayuki
    • Department of Biomaterials Science, Graduate School of Biomedical Sciences, Hiroshima University
    • MORI Kenta
    • Division of Oral Biology and Disease Control, Department of Periodontology, Osaka University Graduate School of Dentistry
    • MURAKAMI Shinya
    • Division of Oral Biology and Disease Control, Department of Periodontology, Osaka University Graduate School of Dentistry
    • POOLTHONG Suchit
    • Department of Operative Dentistry, Faculty of Dentistry, Chulalongkorn University

Abstract

This study aimed to investigate the <i>in vitro </i>biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP<sup>®</sup>) containing 2.5% magnesium carbonate apatite (MgCO<sub>3</sub>Ap). MgCO<sub>3</sub>Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP<sup>®</sup> served as a control. Test and control cements were prepared by encapsulated mixing the powder with Fuji IX-liquid (P/L=3.6:1). Eluates from cements extracted by 1 mL culture medium were collected at day 1, 7 and 14, and used for WST-1 proliferation assay. For ALPase activity, cells were maintained with cements in transwells, harvested and enzyme activity was measured at day 1, 4, 7, 14, and 21. We found a higher cell proliferation and increased ALPase activity by pulp cells in the test group compared to the control. This suggests the potential of GIC containing this novel biological apatite as a restorative material for pulp-dentin regeneration.

Journal

  • Dental Materials Journal

    Dental Materials Journal 31(5), 772-778, 2012-09-01

    The Japanese Society for Dental Materials and Devices

References:  23

Codes

  • NII Article ID (NAID)
    10030875581
  • NII NACSIS-CAT ID (NCID)
    AA10443149
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    02874547
  • Data Source
    CJP  J-STAGE 
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