5-Azacytidine-induced Apoptosis in Lymphoid and Hematopoietic Organs of Adult Mice

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Author(s)

    • HOSSAIN Md. Mokbul
    • Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • NAKAYAMA Hiroyuki
    • Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • SHINOZUKA Junko
    • Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • KATAYAMA Kei-ichi
    • Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • SUZUKI Kazuhiko
    • Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo
    • DOI Kunio
    • Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo

Abstract

The present study deals with the effects of 5-azacytidine (5AzC) on the lymphoid and hematopoietic tissues in adult mice. Seven-week-old, male BALB/c mice were injected with 5AzC (100 mg/kg) intraperitoneally, and the thymus, spleen, and bone marrow were examined for cellular proliferative activity and apoptotic changes. In 5AzC-treated mice, the number of proliferative cell nuclear antigen (PCNA)-positive cells began to decrease at 6 hours after treatment (AT) in the thymic cortex and bone marrow, and at 12 hours AT in the splenic white pulp. The number of TUNEL-positive cells strikingly increased up to 24 hours AT in the thymic cortex, to 12 hours AT in the splenic white pulp, and to 6 hours AT in the bone marrow, and decreased thereafter in all tissues. The number of immature myeloid and erythroid cells in the bone marrow decreased after 5AzC-treatment. Electron microscopic study revealed nuclear condensation and fragmentation in pyknotic or karyorrhectic thymic lymphocytes. DNA electrophoresis of the thymus and bone marrow from 5AzC-treated mice at 12 hours AT showed a typical ladder formation. These findings indicate the apoptotic effect of 5AzC on dividing lymphoid and hematopoietic cells even in adult mice.

Journal

  • Journal of Toxicologic Pathology

    Journal of Toxicologic Pathology 13(4), 231-236, 2000-12-20

    JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    10030906524
  • NII NACSIS-CAT ID (NCID)
    AA12022786
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09149198
  • Data Source
    CJP  CJPref  J-STAGE 
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