空気中の変異原性物質の個人曝露に及ぼす喫煙及び受動喫煙の影響 Effects of Cigarette Smoking on the Personal Exposure to Mutagens in Air
生活環境における発がん関連物質への曝露実態に関する研究の一環として, これまでに作成したいくつかの手法 (個人曝露空気のニコチン濃度, PAH濃度及び変異原性, 尿の変異原性, 尿のニコチン・コチニン濃度及び変異原性など) を組み合わせて小規模な実態調査を実施し, 空気中の発がん関連物質の個人曝露に及ぼす喫煙及び受動喫煙の影響について検討した。その結果, (1) 個人曝露空気のニコチン濃度は, 個人曝露空気の変異原性や尿のコチニン濃度, 尿の変異原性と良好な相関を示すこと, (2) 尿の変異原性と個人曝露空気のピレン濃度は他のPAH濃度と比較して良好な相関を示すこと, また (3) 尿のコチニン濃度とも良好な相関が認められるものの, コチニン濃度の低い非喫煙者を対象とした場合, 相関係数の低下が認められることなどが判った。
Effects of cigarette smoking on the personal exposure to mutagens in air were demonstrated. Airborne particulate and gaseous samples were collected by filters and adsorbent tubes with mini pump for 24 hours. Urine samples were also collected simultaneously for 24 hours. The concentrations of nicotine and 7 polycyclic aromatic hydrocarbons (PAHs) in personal exposure air samples were determined with GC/MS analysis and with HPLC/spectrofluorometric analysis, respectively. The mutagenicity of airborne particulate samples were assayed with <I>Salmonella typhimurium</I> strain TM677 in the absence of S9mix. The concentrations of nicotine and its metabolite cotinine in urine samples were determined with GC/MS analysis. The mutagenicity of Sep-Pak tC<SUB>18</SUB> extracts for urine samples were assayed with <I>Salmonella typhimurium</I> strains TA100, TA98 and YG1024 in the presence and absence of S9mix.<BR>The concentration of nicotine in personal exposure air showed good correlations with the mutagenicity of personal exposure air, with the concentration of cotinine in urine and with the mutagenicities of urine, especially in the presence of S9mix. The correlation of the urinary mutagenicity with the concentration of pyrene in the air were better than those with other PAHs in the air. Also a good correlation was obtained between the concentration of cotinine in urine and the mutagenicities of urine, however their correlations were decreased in the case of non-smokers'.
- 環境化学 : journal of environmental chemistry
環境化学 : journal of environmental chemistry 7(2), 281-289, 1997-06-01