Identification of Phenolic Compounds in Aquilaria crassna Leaves Via Liquid Chromatography-Electrospray Ionization Mass Spectroscopy
In this study, we extracted <i>Aquilaria crassna </i>with aqueous ethanol and water and analyzed the extracts via liquid chromatography diode array detection and electrospray ionization mass spectrometry (LC– ESI–MS) methods. Phenolics were separated using semi-micro HPLC and were identified as iriflophenone 3,5-<i>C</i>-β-diglucoside (1), iriflophenone 3-<i>C</i>-β-glucoside (2), mangiferin (3), iriflophenone 2-<i>O</i>-α-rhamnoside (4), genkwanin 5-<i>O</i>-β-primeveroside (5), genkwanin 5-<i>O</i>-β-glucoside (6), genkwanin 4′-methyl ether 5-<i>O</i>-β-primeveroside (7), and genkwanin (8) via a comparison with authentic samples. The collision-induced dissociation (CID)-MS/MS spectra of these polyphenols and the unknown chromatographic peaks were detected using hybrid ion trap time-of-flight (IT-TOF) mass spectrometry. The results of the present study demonstrated that LC–ESI–MS can be useful for the specific quality control of extracts of extracted <i>A. crassna</i>.
- Food science and technology research
Food science and technology research 18(2), 259-262, 2012-03-01